Protein Info for BWI76_RS22105 in Klebsiella michiganensis M5al

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 549 (545 residues), 762.1 bits, see alignment E=1.8e-233 PF00682: HMGL-like" amino acids 31 to 311 (281 residues), 249.1 bits, see alignment E=5.8e-78 PF08502: LeuA_dimer" amino acids 420 to 549 (130 residues), 69.5 bits, see alignment E=2.7e-23

Best Hits

Swiss-Prot: 61% identical to LEU1_MARMS: 2-isopropylmalate synthase (leuA) from Marinomonas sp. (strain MWYL1)

KEGG orthology group: None (inferred from 90% identity to eae:EAE_01465)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXA1 at UniProt or InterPro

Protein Sequence (558 amino acids)

>BWI76_RS22105 2-isopropylmalate synthase (Klebsiella michiganensis M5al)
MLTQPGEKYRPYPPIALPDRRWPEQQITRAPRWLSTDLRDGNQALAEPMDSARKLQFWDL
LLECGFKEIEVAFPSASQTDFNFVRQLIEERRIPQDVTIQVLTQAREDLILRTFESLRDA
HRATVHLYNATAPLFRERVFGMNKAQVVELATRATRLIRQRCEQQPQTRWQYEYSPETFC
FTEPEFALEICEAVAEIWQPCAERPMIINLPATVEVNTPNVYADQIEYFCRHFSRRADVC
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGVDPRLR
FAEMNRVVEIVESCNQIPVHPRHPWAGSLAYTAFSGSHQDAIKKGFDARRPGDRWEMPYL
PIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPRALQQDFSLHVQRETDTHGKE
MTHNALWQLFRARYGLVPQPPLALLDYQSDSQQDGQLSLQASLRIAGETRRLQSRGNGLL
SAAASGLSGLLKQNIVIKDYHEHTLGERSDSRSVAYIRCVFPNGDSRWGVGIDNDVARAS
LQALCNAMSAGHYSGERK