Protein Info for BWI76_RS21415 in Klebsiella michiganensis M5al

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 transmembrane" amino acids 21 to 48 (28 residues), see Phobius details amino acids 62 to 84 (23 residues), see Phobius details amino acids 96 to 116 (21 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details amino acids 163 to 183 (21 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 275 to 299 (25 residues), see Phobius details amino acids 312 to 331 (20 residues), see Phobius details amino acids 337 to 360 (24 residues), see Phobius details amino acids 371 to 393 (23 residues), see Phobius details amino acids 405 to 423 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 24 to 221 (198 residues), 93 bits, see alignment E=4.1e-30 amino acids 224 to 419 (196 residues), 44 bits, see alignment E=3e-15 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 30 to 418 (389 residues), 477.2 bits, see alignment E=2.2e-147 PF07690: MFS_1" amino acids 64 to 277 (214 residues), 58.2 bits, see alignment E=1.4e-19 amino acids 257 to 421 (165 residues), 42.2 bits, see alignment E=1e-14 PF12832: MFS_1_like" amino acids 261 to 419 (159 residues), 28 bits, see alignment E=2.4e-10

Best Hits

Swiss-Prot: 87% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 94% identity to kpn:KPN_02910)

MetaCyc: 87% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Alpha-ketoglutarate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7Y0 at UniProt or InterPro

Protein Sequence (432 amino acids)

>BWI76_RS21415 MFS transporter (Klebsiella michiganensis M5al)
MTESSISERESQDHGDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
QLLQTAGVFAAGFLMRPIGGWIFGRIADRRGRKTSMLISVCMMCFGSLVIACLPGYATIG
TWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGKKGFYASFQYVTLIGGQLLAVLVV
VALQQILSDEALHAWGWRIPFALGAVLAIVALWLRRQLDETSKQETRALKEAGSFKGLWR
NRKAFIMVLGFTAAGSLSFYTFTTYMQKYLVNTAGMTAGTASMIMTAALFVYMLVQPLFG
ALSDKIGRRTSMLCFGVLVTVFTVPILNALQNVSSPYAAFALVICALLIVSFYTSISGIL
KAEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSAGIEHAFYWYVTVMGAIAFLVS
LTLHRKGKGLRL