Protein Info for BWI76_RS21265 in Klebsiella michiganensis M5al

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1295 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1293 (1290 residues), 2029.9 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 36 to 150 (115 residues), 147.6 bits, see alignment E=2.9e-47 PF18072: FGAR-AT_linker" amino acids 172 to 220 (49 residues), 81.6 bits, see alignment (E = 9.4e-27) PF02769: AIRS_C" amino acids 432 to 588 (157 residues), 109.6 bits, see alignment E=3.4e-35 amino acids 827 to 967 (141 residues), 70.2 bits, see alignment E=4.6e-23 PF13507: GATase_5" amino acids 1039 to 1292 (254 residues), 355.2 bits, see alignment E=3.4e-110

Best Hits

Swiss-Prot: 91% identical to PUR4_ECO57: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O157:H7

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 72% identity to aap:NT05HA_1480)

MetaCyc: 91% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7J1 at UniProt or InterPro

Protein Sequence (1295 amino acids)

>BWI76_RS21265 phosphoribosylformylglycinamidine synthase (Klebsiella michiganensis M5al)
MMEILRGSPALSAFRINKLLARFQAANLPVSTIYAEYVHFADLSAPLSGDDRESLARLLK
YGPNLSSHTPTGKLLLVTPRPGTISPWSSKATDIAHNCGLSQVVRLERGVAYYVEAGSLN
DEQWSLVAAELHDRMMESVFTALEEGEKLFAHHQPTPVTSVDLLGLGRQALIDANLRLGL
ALAEDEIDYLQDAFQKLGRNPNDIELYMFAQANSEHCRHKIFNADWVIDGEKQPKSLFKM
IKNTFEKTPDYVLSAYKDNAAVMEGSAVGRYFADHETGRYDFHQEDAHILMKVETHNHPT
AISPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFEQPWEEDFGKPDRIV
TALDIMTEGPLGGAAFNNEFGRPALNGYFRTYEEKVASHNGEELRGYHKPIMLAGGIGNI
RGEHVQKGEVVVGAKLIVLGGPAMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERR
CQEVIDRCWQLGDANPILFIHDVGAGGLSNAMPELVSDGGRGGKFQLRDILSDEPGMSPL
EIWCNESQERYVLAVAPEQLPLFDELCRRERAPYAVIGEATEALHLSLDDSHFDDRPIDL
PLDVLLGKTPKMTRDVQTLKAQGQSLVRQEITIADAVNRVLHLPTVAEKTFLVTIGDRTV
TGMVARDQMVGPWQIPVANCAVTTASLDSYYGEAMSIGERAPVALLDFAASARLAVGEAL
TNIAATQIGALNRVKLSANWMAAAGHPGEDAGLYEAVKAIGEELCPALGLTIPVGKDSMS
MKTRWQEGTEQREMTSPLSLVISAFARVEDVRRTVTPQLATVDNALLLIDLGKGNNALGA
TALAQVYRQLGDVTADVRDVAQLKGFWDAMQALVAEGKLLAWHDRSDGGLLVTLAEMAFT
GHCGVKVDIAALGEDRLAALFNEELGGVIQVRAADRQAVEALLAAHGLADCVHYLGQATA
GDRFVIEADGHPLFSESRTTLRMWWAETTWQMQRLRDNPECADQEHEAKANDADPGLNVK
LSFDINEDIAAPYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRT
GLADFQALVACGGFSYGDVLGAGEGWAKSILFNERVRDEFATFFHRPQTLALGVCNGCQM
MSNLRELIPGSDLWPRFVRNQSDRFEARFSLVEVTQSPSLLLQGMVGSMMPIAVSHGEGQ
VEVRDGAHLAHLESKGLVALRFVDNFGKVTENYPANPNGSPNGITAVTSESGRATIMMPH
PERVFRTVSNSWHPENWGEDSPWMRIFRNARKQLG