Protein Info for BWI76_RS21105 in Klebsiella michiganensis M5al

Annotation: penicillin-binding protein 1C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 774 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR02073: penicillin-binding protein 1C" amino acids 37 to 774 (738 residues), 837.3 bits, see alignment E=6.6e-256 PF00912: Transgly" amino acids 60 to 226 (167 residues), 173.8 bits, see alignment E=3.7e-55 PF00905: Transpeptidase" amino acids 305 to 520 (216 residues), 41.7 bits, see alignment E=1.3e-14 PF06832: BiPBP_C" amino acids 689 to 770 (82 residues), 42.7 bits, see alignment E=6.5e-15

Best Hits

Swiss-Prot: 77% identical to PBPC_ECOLI: Penicillin-binding protein 1C (pbpC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 86% identity to eae:EAE_00730)

MetaCyc: 77% identical to peptidoglycan glycosyltransferase PbpC (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C" in subsystem Peptidoglycan Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 2.4.2.-

Use Curated BLAST to search for 2.4.1.129 or 2.4.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7S1 at UniProt or InterPro

Protein Sequence (774 amino acids)

>BWI76_RS21105 penicillin-binding protein 1C (Klebsiella michiganensis M5al)
MRVLKRIKRRWRGLAAFIFLLWLLLLAADRLWPLPLHEVTPARVVVAEDGTPLWRFADAQ
GVWRYPVTLADVSPRYLQALIHYEDRWFWDHPGVNPLSIARAAWQDLTSGRVISGGSTLT
MQVARLLDPHPRTFGGKLRQLWRALQLEWHLSKSEILTLYLNRAPFGGTLQGIGAASWAY
LGKPPAQLSYGEAALLAVLPQAPSRLRPDRWPERAQAARDKVLLRMESQGVWPSQPVGEA
LEEPVWLFPRQMPQLAPLFARRALASSRAEKVVTTLDAGLQRQLEDLALNWKSRLPPRSS
LAMIVVDHTNMKVRGWVGSVDIGDDSRFGHIDMVSAIRSPGSVLKPFVYGMALDEGLIHP
ASLLQDVPRRFSDYRPGNFDSGFHGPVSASDALVRSLNLPAVQVLEAYGPKRFAARLRNA
GLPLMLPAGAEPNLSLILGGAGARLADIVAAYSAFARHGKAGQLRITPDSPLVERPLLSP
GAAWIVRRILAGEAQPVPDASLVKVVPLAWKTGTSYGYRDAWAIGVNARYLIGIWTGRPD
GTPVVGQFGFVSAVPLLNQVNNLLLARPAMSRGGLPADPRPSSVSAGTVCWPGGQNLAAG
DANCRRRLATWLLDESQPPTLLLPGQEGIRGIRFPVWLNEKGERVAADCPGAVAKSLDVW
PLPLEPWLPAGERRRARLPAVSADCPPVQTVDAAPLVLSGIREGAVVKRLPGELKVMLPL
QTTGGEGRRWWFLNGEPLTAEGASAILNLERPDSYQLVVMDEAGQIVAANFTVQ