Protein Info for BWI76_RS21065 in Klebsiella michiganensis M5al

Annotation: ribosome biogenesis GTPase Der

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 460 (458 residues), 593.7 bits, E=3.4e-182
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 157 (154 residues), 75.3 bits, E=7.3e-25
  • amino acids 204 to 370 (167 residues), 77.4 bits, E=1.6e-25
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 105.4 bits, E=8.9e-34
  • amino acids 207 to 324 (118 residues), 95.5 bits, E=9.9e-31
• PF02421: FeoB_N
  • amino acids 5 to 157 (153 residues), 58.5 bits, E=2.6e-19
  • amino acids 206 to 368 (163 residues), 47.7 bits, E=5.7e-16
• PF00009: GTP_EFTU
  • amino acids 206 to 372 (167 residues), 44.5 bits, E=6e-15
• PF14714: KH_dom-like
  • amino acids 380 to 460 (81 residues), 93.4 bits, E=3.7e-30

Best Hits

Swiss-Prot: 96% identical to DER_KLEP7: GTPase Der (der) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 98% identity to eae:EAE_00690)

MetaCyc: 92% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A285B7K5 at UniProt or InterPro

Protein Sequence (492 amino acids)

>BWI76_RS21065 ribosome biogenesis GTPase Der (Klebsiella michiganensis M5al)
MIPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEVEGREFICIDTGGI
DGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADSAIAKHLRSREKPTFLVANKT
DGIDADQAIADFWSLGLGEIYPIAASHGRGVTSLLEHVLLPWVDEVNPPEEVDEDAAYWA
QFEAEQNGAEVEEPEDDFNPQDLPIKLAIVGRPNVGKSTLTNRILGEDRVVVYDMPGTTR
DSIYIPMQRDEREYVLIDTAGVRKRGKITDVVEKFSVIKTLQAIEDANVVLLVIDAREGI
SDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQVKETLDFRLGFIDFARVHFISALHG
SGVGNLFESVREAYDSATRRVGTAMLTRIMTMAAEDHQPPLVRGRRVKLKYAHAGGYNPP
IVVIHGNQVKDLPDSYKRYLMNYFRKSLEVMGTPIRIQFKEGENPFANKRNTLTPNQMRK
RKRLIKHIKKSK