Protein Info for BWI76_RS21025 in Klebsiella michiganensis M5al

Annotation: GMP synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00888: GMP synthase (glutamine-hydrolyzing), N-terminal domain
  • amino acids 10 to 205 (196 residues), 279 bits, E=1.4e-87
• PF00117: GATase
  • amino acids 11 to 199 (189 residues), 156.5 bits, E=1.6e-49
• PF07722: Peptidase_C26
  • amino acids 75 to 183 (109 residues), 34.5 bits, E=4.6e-12
• TIGR00884: GMP synthase (glutamine-hydrolyzing), C-terminal domain
  • amino acids 212 to 525 (314 residues), 515.5 bits, E=5.2e-159
• PF02540: NAD_synthase
  • amino acids 216 to 256 (41 residues), 29.2 bits, 1.3e-10
• PF03054: tRNA_Me_trans
  • amino acids 229 to 262 (34 residues), 23 bits, see alignment (E = 1.4e-08)
• PF00958: GMP_synt_C
  • amino acids 433 to 524 (92 residues), 147.3 bits, E=2.9e-47

Best Hits

Swiss-Prot: 97% identical to GUAA_ENT38: GMP synthase [glutamine-hydrolyzing] (guaA) from Enterobacter sp. (strain 638)

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 95% identity to ecc:c3026)

MetaCyc: 95% identical to GMP synthetase (Escherichia coli K-12 substr. MG1655)
GMP synthase (glutamine-hydrolyzing). [EC: 6.3.5.2]; 6.3.5.2 [EC: 6.3.5.2]

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Sequence Analysis Tools

See A0A285B7I3 at UniProt or InterPro

Protein Sequence (525 amino acids)

>BWI76_RS21025 GMP synthase (glutamine-hydrolyzing) (Klebsiella michiganensis M5al)
MTDNIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEAQIREFNPNGIILSGGP
ESTTEDNSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEASNEREFGYAQVEVQTDSA
LIHGIEDALSADGKPMLDVWMSHGDKVTAIPSDFVTVASTETCPFAIMANEEKRFYGVQF
HPEVTHTRQGLRMLERFVRDICQCEALWTPAKIIDDAIERIRQQVGDDKVILGLSGGVDS
SVTAMLLHRAIGKNLTCVFVDNGLLRLNEAQQVMDMFGDRFGLNIVHVEGEARFLSALAG
ENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQGTIYPDVIESAASATGKAHVIKSHHN
VGGLPKEMKMGLVEPLRELFKDEVRKIGLELGLPYDMLYRHPFPGPGLGVRVLGEVKKEY
CDLLRRADAIFIEELHKADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDF
MTAHWAHLPYDFLGRVSNRIINEVNGISRVVYDISGKPPATIEWE