Protein Info for BWI76_RS20860 in Klebsiella michiganensis M5al

Annotation: aminoglycoside/multidrug transporter subunit AcrD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1037 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 390 (25 residues), see Phobius details amino acids 396 to 415 (20 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 470 to 495 (26 residues), see Phobius details amino acids 541 to 559 (19 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 923 to 947 (25 residues), see Phobius details amino acids 971 to 990 (20 residues), see Phobius details amino acids 1004 to 1026 (23 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1027 (1027 residues), 1404.5 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1035 (1035 residues), 1784.1 bits, see alignment E=0 PF03176: MMPL" amino acids 332 to 514 (183 residues), 29.9 bits, see alignment E=4.4e-11

Best Hits

Swiss-Prot: 92% identical to ACRD_ECOLI: Probable aminoglycoside efflux pump (acrD) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 64% identity to abo:ABO_0964)

MetaCyc: 63% identical to multidrug efflux pump RND permease AcrF (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-367

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7L7 at UniProt or InterPro

Protein Sequence (1037 amino acids)

>BWI76_RS20860 aminoglycoside/multidrug transporter subunit AcrD (Klebsiella michiganensis M5al)
MANFFIDRPIFAWVLAILLCLTGILAILSLPVEQYPELAPPNVRITANYPGASAQTLENT
VTQVIEQNMTGLDNLMYMSSQSSGTGQATVTLSFTAGTDPDEAVQQVQNQLQSAMRKLPQ
AVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVASNIQDPLSRVNGVGDIDAYGS
QYSMRIWLDPAKLNSFQMTAKDVTDAISSQNAQIAVGQLGGTPSVDKQALNATINSQSLL
QTPEQFRDITLRVNQDGSEVTLGDVATVEMGAEKYDYLSRYNRQAASGLGVKLASGANEM
ATAERVIDRLNELSQYFPHGLEYKVAYETTSFVKASITDVVKTLLEAIALVFLVMYLFLQ
NFRATLIPTIAVPVVLMGTFAVLYACGYSINTLTMFAVVLAIGLLVDDAIVVVENVERIM
SEEGLSPREATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGTTGAIYRQFSITIVSAMVL
SVLVAMILTPALCATLLKPVHKGETHGQRGFFGWFNRTFNRNASRYETAVGKILHRSLRW
IMIYVLLLGGMVFLFLHLPTSFLPLEDRGMFTTSVQLPSGSTQQQTLKVVQQAEDYFLNN
EKQNVESVFATIGSGPGGNGQNVARMFVRLKDWDQRDPTTGSSFAIIERATKAFNKIKEA
RVFASSPPAISGLGSSAGFDMELEDHAGNGHEALMAARDTLLELAAKNNQLTRVRHNGLD
DSPQLQVDIDQRKAQALGVSIDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRML
PDDINLWYVRNSSGTMVPFSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMT
LMEELAQQLPNGFGLEWTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVM
LVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANELNQKGEDLLTA
TLSACRQRLRPILMTSLAFIFGVLPMATSTGAGSGSQHAVGTGVMGGMISATVLAIFFVP
LFFVLVRRRFPLKERPQ