Protein Info for BWI76_RS20745 in Klebsiella michiganensis M5al

Annotation: AraC family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 PF00165: HTH_AraC" amino acids 250 to 290 (41 residues), 33.2 bits, see alignment 4.3e-12 PF12833: HTH_18" amino acids 262 to 341 (80 residues), 63.1 bits, see alignment E=2.4e-21

Best Hits

Swiss-Prot: 94% identical to EUTR_ECOLI: HTH-type transcriptional regulator eutR (eutR) from Escherichia coli (strain K12)

KEGG orthology group: K04033, AraC family transcriptional regulator, ethanolamine operon transcriptional activator (inferred from 94% identity to eco:b2437)

Predicted SEED Role

"Ethanolamine operon regulatory protein" in subsystem Ethanolamine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7J8 at UniProt or InterPro

Protein Sequence (350 amino acids)

>BWI76_RS20745 AraC family transcriptional regulator (Klebsiella michiganensis M5al)
MKKKTPVNLHHLYHEALPEDVKLTPMVEVDNVHQRRTTDVYEHALTITAWQQIYDQLHPG
KFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSAEI
ATRPGGTEFELSTPDDYTILGVVISEDVITRHAGFLHHPERVLHMLRNQSALEVKEQHKA
ALWGFVQQALATFSENPENLHQPAVRKVLGDNLLLAMGAMLEEAQPIVTAESISHQSYRR
LLSRAREYVLENMSEPLTVLDLCNQLYVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
LISPWSQSATVKDAAMQWGFWHLGQFATDYQQLFAEKPSLTLHQRMRQWA