Protein Info for BWI76_RS20705 in Klebsiella michiganensis M5al

Annotation: thiosulfate transporter subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 15 to 332 (318 residues), 600.3 bits, see alignment E=3.9e-185 PF01547: SBP_bac_1" amino acids 37 to 277 (241 residues), 71 bits, see alignment E=2.8e-23 PF13531: SBP_bac_11" amino acids 38 to 286 (249 residues), 126.5 bits, see alignment E=2.1e-40 PF13343: SBP_bac_6" amino acids 135 to 298 (164 residues), 26.1 bits, see alignment E=8.3e-10

Best Hits

Swiss-Prot: 95% identical to CYSP_ECOLI: Thiosulfate-binding protein (cysP) from Escherichia coli (strain K12)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 96% identity to kva:Kvar_1283)

MetaCyc: 95% identical to thiosulfate/sulfate ABC transporter periplasmic binding protein CysP (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B781 at UniProt or InterPro

Protein Sequence (338 amino acids)

>BWI76_RS20705 thiosulfate transporter subunit (Klebsiella michiganensis M5al)
MAVKSLTKGYLALAASVLLVAQAQATELLNSSYDVSRELFAALNPQFEQQWAKDNGGDKL
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQSRLPNNSSPFYS
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKAKT
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEDQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYSYRVNNPDVMNKLKDKF
PQTDLFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRK