Protein Info for BWI76_RS20665 in Klebsiella michiganensis M5al

Annotation: bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 22 to 279 (258 residues), 166.6 bits, E=3.9e-53
• PF08543: Phos_pyr_kin
  • amino acids 43 to 268 (226 residues), 82.5 bits, E=3.4e-27
• PF00294: PfkB
  • amino acids 152 to 261 (110 residues), 34.7 bits, E=1.3e-12

Best Hits

Swiss-Prot: 74% identical to PDXK_SALTY: Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 86% identity to eae:EAE_00385)

MetaCyc: 69% identical to pyridoxine/pyridoxal/pyridoxamine kinase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylpyrimidine kinase. [EC: 2.7.1.49]; Pyridoxal kinase. [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (8/9 steps found)
• thiamine diphosphate salvage II (5/5 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate salvage IV (yeast) (5/7 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism
• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.35, 2.7.1.49

Use Curated BLAST to search for 2.7.1.35 or 2.7.1.49

Sequence Analysis Tools

See A0A285B752 at UniProt or InterPro

Protein Sequence (288 amino acids)

>BWI76_RS20665 bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase (Klebsiella michiganensis M5al)
MDQQDEIQSVLFSDKSRALQVDIVAVQSQVVYGSVGNSIAIPAIKQHGLRVMAVPTVLFS
NTPHYDTYYGGVIPEEWFAGYLKALEERDALRELRAVTTGYMGSAVQIKRLAQWLQRIRE
QRPELCILVDPVIGDVDSGIYVKAEIPEAYRTHLLPLAQGITPNLFELETLSGLPCHHQQ
EAIIAARSLLSDSLQWVVITSAPGADSATINVLVVSAESVEVIEHPRVETDLKGTGDLFC
AELASGLVCGIPLGDAAKAAAQRVLAVMTWTEEQGCDELILPPRGEGV