Protein Info for BWI76_RS20650 in Klebsiella michiganensis M5al

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 2 to 566 (565 residues), 890.8 bits, see alignment E=1.7e-272 PF05524: PEP-utilisers_N" amino acids 3 to 126 (124 residues), 111.8 bits, see alignment E=3.6e-36 PF00391: PEP-utilizers" amino acids 152 to 222 (71 residues), 82 bits, see alignment E=3e-27 PF02896: PEP-utilizers_C" amino acids 250 to 541 (292 residues), 441.8 bits, see alignment E=1.7e-136

Best Hits

Swiss-Prot: 96% identical to PT1_SALTI: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Salmonella typhi

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 98% identity to kpn:KPN_02763)

MetaCyc: 95% identical to PTS enzyme I (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate--protein phosphotransferase. [EC: 2.7.3.9]

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7H9 at UniProt or InterPro

Protein Sequence (575 amino acids)

>BWI76_RS20650 phosphoenolpyruvate--protein phosphotransferase (Klebsiella michiganensis M5al)
MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLNGRTKASAQLEVIKTK
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAANEVIEGQATALEELDD
EYLKERAADVRDIGKRLLRNILGLAIIDLSAIQDEVILVAADLTPSETAQLNLNKVLGFI
TDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNGDYLVLDAVNNQVLVNPSDQQIEELR
NLQAQVAEEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL
FMDRDSLPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAV
RIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVRALKKEIEIYKQELRDEGKAF
DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS
VLTLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
NFEDAKVLAEQALAQPTTDELMTLVNKFIEEKTIC