Protein Info for BWI76_RS20605 in Klebsiella michiganensis M5al

Annotation: purine-nucleoside phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01699: xanthosine phosphorylase
  • amino acids 27 to 274 (248 residues), 508.5 bits, E=3e-157
• TIGR01697: inosine/guanosine/xanthosine phosphorylase family
  • amino acids 27 to 272 (246 residues), 354.7 bits, E=2.4e-110
• PF01048: PNP_UDP_1
  • amino acids 28 to 274 (247 residues), 154 bits, E=2.2e-49

Best Hits

Swiss-Prot: 88% identical to XAPA_ECOLI: Purine nucleoside phosphorylase 2 (xapA) from Escherichia coli (strain K12)

KEGG orthology group: K03815, xanthosine phosphorylase [EC: 2.4.2.-] (inferred from 94% identity to cko:CKO_00389)

MetaCyc: 88% identical to xanthosine phosphorylase (Escherichia coli K-12 substr. MG1655)
Purine-nucleoside phosphorylase. [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.1, 2.4.2.15]; 2.4.2.- [EC: 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Xanthosine phosphorylase (EC 2.4.2.1)" in subsystem Xanthosine utilization (xap region) (EC 2.4.2.1)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• ureide biosynthesis (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• nucleoside and nucleotide degradation (halobacteria) (3/6 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-, 2.4.2.1

Use Curated BLAST to search for 2.4.2.- or 2.4.2.1 or 2.4.2.15

Sequence Analysis Tools

See A0A285B758 at UniProt or InterPro

Protein Sequence (277 amino acids)

>BWI76_RS20605 purine-nucleoside phosphorylase (Klebsiella michiganensis M5al)
MSDTLFSQNPWYSADIIRGYKPDFTPRVAFILGSGLGALAEQIEDAVAISYEKLPGFPVS
TVHGHAGELVLGNLAGVPVACMKGRGHFYEGRGMPVMTDAIRTLKLLGCELLFCTNAAGS
LRPEVGPGSLVALSDHINTMPGTPMVGPNDERFGERFFSLANAYDADYRAVLQSVAAEEG
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVITARHCGLKVVAVSAITNL
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA