Protein Info for BWI76_RS20500 in Klebsiella michiganensis M5al

Annotation: signal transduction histidine kinase LytS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 45 to 64 (20 residues), see Phobius details amino acids 75 to 99 (25 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 140 to 163 (24 residues), see Phobius details amino acids 173 to 193 (21 residues), see Phobius details PF07694: 5TM-5TMR_LYT" amino acids 29 to 192 (164 residues), 145.3 bits, see alignment E=3.4e-46 PF07155: ECF-ribofla_trS" amino acids 47 to 181 (135 residues), 30.3 bits, see alignment E=1e-10 PF01590: GAF" amino acids 226 to 345 (120 residues), 26.8 bits, see alignment E=1.7e-09 PF06580: His_kinase" amino acids 359 to 438 (80 residues), 88.1 bits, see alignment E=9.6e-29 PF02518: HATPase_c" amino acids 456 to 552 (97 residues), 38.4 bits, see alignment E=3.7e-13

Best Hits

Swiss-Prot: 89% identical to YPDA_ECO57: Sensor histidine kinase YpdA (ypdA) from Escherichia coli O157:H7

KEGG orthology group: K02478, two-component system, LytT family, sensor kinase [EC: 2.7.13.3] (inferred from 93% identity to kpn:KPN_02736)

MetaCyc: 89% identical to sensor histidine kinase PyrS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Autolysis histidine kinase LytS" in subsystem Murein hydrolase regulation and cell death

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7P4 at UniProt or InterPro

Protein Sequence (565 amino acids)

>BWI76_RS20500 signal transduction histidine kinase LytS (Klebsiella michiganensis M5al)
MHEIFTMLLAVFDRAALMLICLFFLIRLRLFRELLHKSAHTPKELLAVTAIFSLFALFST
WSGVPVEGSLVNVRIIAVMSGGILFGPWVGAIVGAIAGIHRYLIDIDGVTAVPCFITSIV
AGLLSGFISRKVPKAQRWKAGILAGMLCETLTMILVVVWAPSLALGIDIVSKIGIPMILG
SVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLPLFRHVNSESLRQVCDIIRRDIN
ADAVAITNTQQVMAYVGVGEINYRDNDDVISPTTQQAIRYGKIIIKNNDEAHRTPEIHSM
LVIPLWEKGVVTGTLKIYYCHAHRITSSLQEMAIGLSQIISTQLEVSRAEQLREMANKAE
LRALQSKINPHFLFNALNAISSSIRMNPDTARQLIFNLSRYLRYNIELKDDEQIDIKREL
YQIKDYIAIEQARFGDKLTVIYDIDDEVSFMIPSLLIQPLVENAIVHGIQPCKGKGVVTI
GINECGNRVRISVRDTGNGIDPAVVARVEADEMPGNKIGLLNVHHRVKLLYGEGLHIRRL
TPGTEIAFYVPTQYAPQVATESLLS