Protein Info for BWI76_RS20460 in Klebsiella michiganensis M5al

Annotation: acetyl-CoA C-acyltransferase FadI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02446: acetyl-CoA C-acyltransferase FadI
  • amino acids 7 to 436 (430 residues), 854.3 bits, E=1.9e-261
• PF00108: Thiolase_N
  • amino acids 15 to 288 (274 residues), 196.9 bits, E=4.3e-62
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 17 to 434 (418 residues), 433.8 bits, E=5.7e-134
• PF02803: Thiolase_C
  • amino acids 297 to 434 (138 residues), 117.7 bits, E=2.6e-38

Best Hits

Swiss-Prot: 94% identical to FADI_KLEP3: 3-ketoacyl-CoA thiolase (fadI) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 93% identity to eae:EAE_24670)

MetaCyc: 88% identical to 3-ketoacyl-CoA thiolase FadI (Escherichia coli K-12 substr. MG1655)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

• oleate β-oxidation (32/35 steps found)
• fatty acid β-oxidation I (generic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (8/10 steps found)
• fatty acid salvage (5/6 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• glycerol degradation to butanol (12/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• polyhydroxybutanoate biosynthesis (2/3 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• ketolysis (1/3 steps found)
• 3-phenylpropanoate degradation (6/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• valproate β-oxidation (5/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• isopropanol biosynthesis (engineered) (2/5 steps found)
• L-glutamate degradation VII (to butanoate) (7/12 steps found)
• 2-deoxy-D-ribose degradation II (4/8 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• ethylbenzene degradation (anaerobic) (1/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
• ketogenesis (1/5 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
• mevalonate pathway II (haloarchaea) (2/7 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (9/17 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 4-ethylphenol degradation (anaerobic) (1/6 steps found)
• 2-methylpropene degradation (2/8 steps found)
• isoprene biosynthesis II (engineered) (2/8 steps found)
• mevalonate pathway III (Thermoplasma) (2/8 steps found)
• mevalonate pathway IV (archaea) (2/8 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• 4-oxopentanoate degradation (1/9 steps found)
• ethylmalonyl-CoA pathway (2/11 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation I (aerobic) (7/25 steps found)
• sitosterol degradation to androstenedione (1/18 steps found)
• platensimycin biosynthesis (6/26 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• superpathway of ergosterol biosynthesis I (4/26 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (23/56 steps found)
• superpathway of L-lysine degradation (12/43 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
• superpathway of cholesterol biosynthesis (4/38 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

See A0A285B710 at UniProt or InterPro

Protein Sequence (436 amino acids)

>BWI76_RS20460 acetyl-CoA C-acyltransferase FadI (Klebsiella michiganensis M5al)
MSQALPLVTRQGDRIAIVSGLRTPFARQATAFHGVPAVDLGKLVVGEMLARSTIPADIIE
QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
GIAGGADSSSVLPIGVSKKLARTLVDANKARTLGQRLKLFSRLRFRDLLPVPPAVAEYST
GLRMGDTAEQMAKTWGITRERQDALALRSHQLAAKAWEEGKLSAEVMTAYTPPFREPLEQ
DNNIRKNSTLADYQKLRPAFDRQHGSVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
LRSYAFTAIDVRQDMLLGPAWSTPLALERAGLTLADLTLIDMHEAFAAQTLANLHCMASD
RFAREVLGRSQAIGEVDESKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
ACAAGGLGSAMVLEAE