Protein Info for BWI76_RS20410 in Klebsiella michiganensis M5al

Annotation: bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 transmembrane" amino acids 267 to 284 (18 residues), see Phobius details PF05430: Methyltransf_30" amino acids 118 to 243 (126 residues), 167.5 bits, see alignment E=1.1e-53 PF01266: DAO" amino acids 266 to 628 (363 residues), 186.5 bits, see alignment E=1.1e-58 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 266 to 658 (393 residues), 519.5 bits, see alignment E=3.1e-160

Best Hits

Swiss-Prot: 83% identical to MNMC_KLEP7: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 83% identity to kpn:KPN_02714)

MetaCyc: 76% identical to fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase (Escherichia coli K-12 substr. MG1655)
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase. [EC: 2.1.1.61]; RXN0-7081 [EC: 2.1.1.61]

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61)" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B702 at UniProt or InterPro

Protein Sequence (662 amino acids)

>BWI76_RS20410 bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC (Klebsiella michiganensis M5al)
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLNERFPGHPRPFF
VVAESGFGTGLNFLTLWQAFDVFHRENPQATLQRLHFISFEKFPLKADDLRLAHQRWPEL
ASWAEQLQAQWPLPIAGCHRLLLDEGRVTLDLWFGDINELTNELDDSLNRQVDAWFLDGF
APAKNPDMWTAELFAAMARLARPGGTLATFTSAGFVRRGLQEAGFSMHKRKGFGRKREML
TGEMLQTLEIPARAPWFARSGTEQRDAAIIGGGIASALLALALLRRGWQVTLYCADSAPA
DGASGNRQGALYPLLSQHDPALARFFPAAFTFARRLYDTLPVMFDHEWCGVTQLGWDVKS
AQKIAQMIELNLPPEIAVAVDAEAAEQQTGVETGCGGIAYPAGGWLCPQQLTAELLALAA
TRGLRVHYDYPVEALSADNSGWQLNQRQYHEVVILANGHRLTHFIQTEHLPMYPVAGQVS
HIPTTPGLSKLRQVLCYNGYLTPQNPHNQQHCIGASYHRGQADAAFSADDQQQNRQRLID
CFPQAEWTREVDVSANQSRSGVRCATRDHLPMVGNVPDYDATLSAYASLAERQEQAGEAP
VYRNLYMLGALGSRGLCSAPLAAEILASQMSNEPLPMDKETLAALNPNRLWVRKLLKGKA
VK