Protein Info for BWI76_RS20365 in Klebsiella michiganensis M5al

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 32 to 413 (382 residues), 358.6 bits, E=2.9e-111
• PF08245: Mur_ligase_M
  • amino acids 54 to 196 (143 residues), 30.4 bits, E=3.7e-11
• PF02875: Mur_ligase_C
  • amino acids 287 to 354 (68 residues), 32.1 bits, E=1.2e-11

Best Hits

Swiss-Prot: 81% identical to FOLC_ECOLI: Dihydrofolate synthase/folylpolyglutamate synthase (folC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 86% identity to eae:EAE_24575)

MetaCyc: 81% identical to bifunctional folylpolyglutamate synthetase / dihydrofolate synthetase (Escherichia coli K-12 substr. MG1655)
Tetrahydrofolate synthase. [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; 6.3.2.17 [EC: 6.3.2.17]; Dihydrofolate synthase. [EC: 6.3.2.17, 6.3.2.12]

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See A0A285B790 at UniProt or InterPro

Protein Sequence (422 amino acids)

>BWI76_RS20365 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Klebsiella michiganensis M5al)
MEKQPIPQAASPLATWLSYLEHLHSKTIDLGLARVSEVAKQMAVLKPAPFVFTVAGTNGK
GTTCRTLESALMAAGYKVGVYSSPHLVRYTERVRVQGEELTELAHTTSFAEIEAARGEIS
LSYFEFGTLSALWLFQQARLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPD
RESIGREKAGIFRAGKAAIVGEPDMPLTIAQVADEKGARLLRRDVDWRYEAGEQGWSFYD
RQGALTDLPLPQVPMPNAATAIAALRASGLKVSEQAIRDGVQRAMLPGRFQIISEAPRTI
LDVAHNPHAAAYLAGRLRTLSKTGRVLAVIGMLHDKDIAGTLANLQTEVDDWYCAPLEGP
RGATAEQLLEHLRTGKVYISVAQAWHAAMADARPEDTVLVCGSFHTVAHVMEEIDAGRIG
GE