Protein Info for BWI76_RS20345 in Klebsiella michiganensis M5al

Annotation: 3-octaprenyl-4-hydroxybenzoate carboxy-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02441: Flavoprotein
  • amino acids 2 to 173 (172 residues), 141.1 bits, E=1.3e-45
• TIGR00421: polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases
  • amino acids 3 to 186 (184 residues), 231.8 bits, E=2.4e-73

Best Hits

Swiss-Prot: 89% identical to UBIX_ECOLI: Flavin prenyltransferase UbiX (ubiX) from Escherichia coli (strain K12)

KEGG orthology group: K03186, 3-octaprenyl-4-hydroxybenzoate carboxy-lyase UbiX [EC: 4.1.1.-] (inferred from 93% identity to kpu:KP1_3948)

MetaCyc: 89% identical to flavin prenyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-16937 [EC: 2.5.1.129]

Predicted SEED Role

"3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-)" in subsystem Ubiquinone Biosynthesis (EC 4.1.1.-)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of ubiquinol-8 biosynthesis (early decarboxylation) (12/12 steps found)
• ubiquinol-8 biosynthesis (early decarboxylation) (8/8 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Fluorene degradation
• Phenylalanine metabolism
• Phenylpropanoid biosynthesis
• Purine metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.-

Use Curated BLAST to search for 2.5.1.129 or 4.1.1.-

Sequence Analysis Tools

See A0A285B6Z2 at UniProt or InterPro

Protein Sequence (189 amino acids)

>BWI76_RS20345 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (Klebsiella michiganensis M5al)
MKRLIIGISGASGAIYGVRLLQVLREVPDVETHLILSQAARQTLALETDFSLREVQALAD
VVHDARDIAASISSGSFKTAGMVILPCSIKTLSGIVHSYTDGLLTRAADVVLKERRPLVL
CVRETPFHLGHLRLLVQAAEMGAVIMPPVPAFYHRPKTLDDVINQTVNRVIDQFDISLPE
DLFTRWQGA