Protein Info for BWI76_RS20280 in Klebsiella michiganensis M5al

Annotation: phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 183 PF12850: Metallophos_2" amino acids 1 to 161 (161 residues), 83.4 bits, see alignment E=1.9e-27 PF00149: Metallophos" amino acids 1 to 115 (115 residues), 48.7 bits, see alignment E=1.3e-16 TIGR00040: phosphodiesterase, MJ0936 family" amino acids 1 to 167 (167 residues), 146.1 bits, see alignment E=4.8e-47

Best Hits

Swiss-Prot: 80% identical to YFCE_ECO57: Phosphodiesterase YfcE (yfcE) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 90% identity to eae:EAE_24485)

Predicted SEED Role

"FIG002292: Phosphodiesterase yfcE (EC 3.1.4.-)" (EC 3.1.4.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.-

Use Curated BLAST to search for 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7A2 at UniProt or InterPro

Protein Sequence (183 amino acids)

>BWI76_RS20280 phosphodiesterase (Klebsiella michiganensis M5al)
MKLMFASDIHGSLPAAERVLELFARSGAQWLVILGDVLNHGPRNALPEGYAPAQVAEALN
RVAERVIAVRGNCDSEVDQMLLHFPITAPWQQVLSERTRLFLTHGHLYSPDNLPPLAAGD
VLAYGHTHIPVAEKRGEIFLFNPGSVSIPKGGFTASYGLLNEGQLQVLALDDNQVIAEVA
IYP