Protein Info for BWI76_RS20165 in Klebsiella michiganensis M5al

Annotation: NADH-quinone oxidoreductase subunit G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 PF13510: Fer2_4" amino acids 3 to 77 (75 residues), 42.8 bits, see alignment E=1.3e-14 PF00111: Fer2" amino acids 4 to 71 (68 residues), 31 bits, see alignment 5.7e-11 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 655 (652 residues), 674.3 bits, see alignment E=1.1e-206 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 124 (37 residues), 56.8 bits, see alignment (E = 3.8e-19) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 49.1 bits, see alignment 1.4e-16 PF00384: Molybdopterin" amino acids 281 to 637 (357 residues), 58.1 bits, see alignment E=2.5e-19 PF01568: Molydop_binding" amino acids 827 to 901 (75 residues), 32.5 bits, see alignment E=2.3e-11

Best Hits

Swiss-Prot: 91% identical to NUOG_SALTY: NADH-quinone oxidoreductase subunit G (nuoG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_24405)

MetaCyc: 66% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B787 at UniProt or InterPro

Protein Sequence (908 amino acids)

>BWI76_RS20165 NADH-quinone oxidoreductase subunit G (Klebsiella michiganensis M5al)
MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTR
GRLVMSCMTPASDGTFISIDDAEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVM
TGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRYYKDYADGTDLGVYGAHDNVY
FGRPEDGTLESEFSGNLVEVCPTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNISPG
ERYGELRRIENRYNGTVNRYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDLITLNAEQAMQ
GAADILRQSKKVIGIGSPRASLESNFALRELVGVGNFYTGIAKGEQERLQMMLKVLREGG
IHTPALREIESYDAVLILGEDITQTGARIALAVRQAVKGKAREMAAAQKVADWQIAAILN
IGQRAKHPLFVTNIDDTRLDDIAAWTYRAPVEDQARLGFAIAHALDESAPAVEGLSGELK
GKIDVIVQALAGAKKPLIVSGTNAGSIEVIQAAANVAKALKGRGADVGVTMVARAVNSVG
LGMIGGGSLEEALSELETGAADAVIVLENDLHRHASAARVDAALAKAPLVMVVDHQRTAI
MEKAHLVLSAASFAESDGTVVNNEGRAQRFFQVYDPAYYDAKTVMLESWRWLHSLHSTVN
NRQVDWTQLDHVIDAAVAALPQLAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANIS
VHEPRQPQDKDTMFAFSMEGNNQPSAPRSQIPFAWAPGWNSPQAWNKFQDEVGGKLRHGD
PGVRLFEASASGLEYFTAVPASFQAEDGKWRIAPYYHLFGSDELSQRSPVFQSRMPQPYV
KLNPADAAKLGVNEGAMVSLRVDGQALSLPLVISAGLTAGQVGLPMGMPGIAPVLTGARI
DTLQEAKA