Protein Info for BWI76_RS20130 in Klebsiella michiganensis M5al

Annotation: NADH-quinone oxidoreductase subunit N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 9 to 475 (467 residues), 482.1 bits, E=1e-148
• PF00361: Proton_antipo_M
  • amino acids 122 to 420 (299 residues), 294.3 bits, E=4.9e-92

Best Hits

Swiss-Prot: 98% identical to NUON_KLEP7: NADH-quinone oxidoreductase subunit N (nuoN) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 97% identity to kva:Kvar_1387)

MetaCyc: 93% identical to NADH:quinone oxidoreductase subunit N (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• nitrate reduction VIII (dissimilatory) (2/2 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See A0A285B6X9 at UniProt or InterPro

Protein Sequence (485 amino acids)

>BWI76_RS20130 NADH-quinone oxidoreductase subunit N (Klebsiella michiganensis M5al)
MTITPQQLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVLGLNAALISLWFVGQNGA
MDVTPMIRVDGYSMLYTGLVLLASLATCTFAYPWLEGYKDNKEEFYLLVLIAALGGILLA
GANHLAALFLGIELISLPLFGLVGYAFRQKRSLEASIKYTILSAVASSFLLFGMALVYAN
SGNLSFLALGKSLTDGMLHEPLLLAGLGLMIVGLGFKLSLVPFHLWTPDVYQGAPAPVST
FLATASKIAIFGVVMRLFLYMPVGGSEAVRVVLGLIAFASIIFGNLMALSQTNIKRLLGY
SSISHLGYLLVALIALQSGEMSMEAVGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLY
SYRGLFWHRPILSAVMTVMMLSLAGIPMTLGFIGKFYVLAVGVHAHLWWLVGAVVLGSAI
GLYYYLRVAVSLYLSAPEKLNRDAPSNWQYSAGGIVVLISALLVLVLGVWPQPLISIVQL
ATPLM