Protein Info for BWI76_RS20080 in Klebsiella michiganensis M5al
Annotation: isochorismate synthase MenF
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 81% identity to eae:EAE_24345)Predicted SEED Role
"Menaquinone-specific isochorismate synthase (EC 5.4.4.2)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 5.4.4.2)
MetaCyc Pathways
- superpathway of chorismate metabolism (54/59 steps found)
- superpathway of menaquinol-8 biosynthesis I (10/10 steps found)
- superpathway of demethylmenaquinol-8 biosynthesis I (9/9 steps found)
- 2-carboxy-1,4-naphthoquinol biosynthesis (7/7 steps found)
- superpathway of demethylmenaquinol-6 biosynthesis I (8/9 steps found)
- superpathway of demethylmenaquinol-9 biosynthesis (8/9 steps found)
- 2,3-dihydroxybenzoate biosynthesis (3/3 steps found)
- superpathway of menaquinol-10 biosynthesis (8/10 steps found)
- superpathway of menaquinol-11 biosynthesis (8/10 steps found)
- superpathway of menaquinol-12 biosynthesis (8/10 steps found)
- superpathway of menaquinol-13 biosynthesis (8/10 steps found)
- superpathway of menaquinol-6 biosynthesis (8/10 steps found)
- superpathway of menaquinol-7 biosynthesis (8/10 steps found)
- superpathway of menaquinol-9 biosynthesis (8/10 steps found)
- salicylate biosynthesis I (2/2 steps found)
- salicylate biosynthesis II (2/3 steps found)
- enterobactin biosynthesis (7/11 steps found)
- vibriobactin biosynthesis (4/9 steps found)
- bacillibactin biosynthesis (6/12 steps found)
- superpathway of phylloquinol biosynthesis (8/15 steps found)
KEGG Metabolic Maps
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of siderophore group nonribosomal peptides
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 5.4.4.2
Use Curated BLAST to search for 5.4.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B6W9 at UniProt or InterPro
Protein Sequence (441 amino acids)
>BWI76_RS20080 isochorismate synthase MenF (Klebsiella michiganensis M5al) MWGFYSVEVRVLSVSTALESLYRCLDEAFPPSPGTRIYDISFALNDAFDPLLWCNHQRQW PQFYWQQRSGDEELAALGAVKTFASLTQAHDFLRQQGRDDLRICGLNAFDPQQGLLLLPR LEWRRLGGSATLRLVLSSESSLRDDARAAREFLDSLTAKQAQATAMPSLLSEQHFPAYPQ WRALITQATQAIAAGEMDKVVLARATDLHFAAPLNAGSIMAASRRSNLNCFHFLMAFTPR EAFVGSTPERLWRRRGALLRTEALAGTVASHADDRQAQRLGNWLMGDDKNQRENMLVVED ICQRLQGETRALEVLPPQIVRLRKVQHLRRCIWAELDKPDDARCLHLLQPTAAVAGLPRQ VALDFITRNELFTRGWYAGSAGYLSLAQSEFCVSLRSAKVENHTLRLYAGAGIVSGSDPE QEWQEIENKAAGLRSLLLTET