Protein Info for BWI76_RS19920 in Klebsiella michiganensis M5al

Annotation: hybrid sensor histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 transmembrane" amino acids 16 to 40 (25 residues), see Phobius details amino acids 315 to 337 (23 residues), see Phobius details PF00512: HisKA" amino acids 470 to 534 (65 residues), 64.2 bits, see alignment 1.9e-21 PF02518: HATPase_c" amino acids 581 to 690 (110 residues), 107.6 bits, see alignment E=9.8e-35 PF09456: RcsC" amino acids 709 to 799 (91 residues), 116.1 bits, see alignment E=1.1e-37 PF00072: Response_reg" amino acids 827 to 935 (109 residues), 101.1 bits, see alignment E=8.6e-33

Best Hits

Swiss-Prot: 82% identical to RCSC_ECOLI: Sensor histidine kinase RcsC (rcsC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to eae:EAE_24225)

MetaCyc: 82% identical to histidine kinase RcsC (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Two-component sensor protein RcsC (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6T4 at UniProt or InterPro

Protein Sequence (948 amino acids)

>BWI76_RS19920 hybrid sensor histidine kinase/response regulator (Klebsiella michiganensis M5al)
MKYLASFHNTLKVSRYLFRALAVLLWVLVAFVSVFYIVNALHDRESEIRQEFNLNADQAQ
RYIQRTADVIKELKYVAGNRPMDGGEGGDTPVQRGDIAIPNFEPLYPDSDCALMSTTWRN
SLQSLAWFMRYWRDNFSTAYDLNRIFLIGSDNLCMANFGLRDIPLERDHALKVLHQRIEQ
YRDAPQNERGNNLFWISQGVRPGVGYFYALTPIYVANRLQAMLGVEQTIRMESFFTPGSL
PMNVTILDDNGQSLISLAGSDSNIKPDAKWMQERLWFGYTAGFRELAMKKNLPPSSLSIV
YSVPVDQVLERIRMLILNAVVLNLLTGVLLFVLARMYERRIFIPAENDAQRLEEHEQFNR
KIVASAPVGICILRTLDGTNILSNELAHNYLNMLTHEDRQRLTQIICGQQVNFVDVLTSN
NTNLQISFVHSRYRNENVAICVLVDVSARVKMEESLQEMAQAAEQASQSKSMFLATVSHE
LRTPLYGIIGNLDLLQTKELPKGVDRLVTAMNNSSSLLLKIISDILDFSKIESEQLKIEP
REFSPREVMNHISANYLPLVVRKQLGLYCFIEPDVPQLMSGDPMRLQQVISNLLSNAIKF
TDTGCIILHVERAGDYLQISVRDTGEGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAI
CEKLISMMDGDIAVETEPGMGSRFTIRIPLYGVQNTPPLLIDGLAEKCCWLAIDNSSLAL
FVEQLLNYHGIPVRRYAGQAPGAEDILLTDSEAREAWPGGAMVIFCRRHIGIPQERAPGE
WLHSVATPHELLPLLGRIFHIAVASAENGPALSAADAQTTSNDDMMILVVDDHPINRRLL
ADQLGSLGYQCVTANDGVDALNVLSKQHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVI
GVTANALAEEKQRCLESGMDSCLSKPVTLDIIKQTLTVYAARVRKGRE