Protein Info for BWI76_RS19895 in Klebsiella michiganensis M5al

Annotation: bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 PF02805: Ada_Zn_binding" amino acids 12 to 75 (64 residues), 101.6 bits, see alignment E=4.9e-33 PF00165: HTH_AraC" amino acids 98 to 133 (36 residues), 42.2 bits, see alignment 1.6e-14 PF12833: HTH_18" amino acids 105 to 182 (78 residues), 61.8 bits, see alignment E=1.6e-20 PF02870: Methyltransf_1N" amino acids 196 to 266 (71 residues), 38.1 bits, see alignment E=5.8e-13 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 269 to 348 (80 residues), 116.6 bits, see alignment E=1.9e-38 PF01035: DNA_binding_1" amino acids 270 to 349 (80 residues), 114.1 bits, see alignment E=5.3e-37

Best Hits

Swiss-Prot: 65% identical to ADA_ECOLI: Bifunctional transcriptional activator/DNA repair enzyme Ada (ada) from Escherichia coli (strain K12)

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 77% identity to kva:Kvar_1419)

MetaCyc: 65% identical to DNA-binding transcriptional dual regulator / DNA repair protein Ada (Escherichia coli K-12 substr. MG1655)
2.1.1.M37 [EC: 2.1.1.M37]; Methylated-DNA--[protein]-cysteine S-methyltransferase. [EC: 2.1.1.M37, 2.1.1.63]; 2.1.1.63 [EC: 2.1.1.M37, 2.1.1.63]

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63 or 2.1.1.M37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6X4 at UniProt or InterPro

Protein Sequence (354 amino acids)

>BWI76_RS19895 bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase (Klebsiella michiganensis M5al)
MKPIMIDTDERRWQAVCERDARADGQFVFAVLTTGVCCRPTCRSRRALRENVRFYPDVAT
AQAEGFRPCKRCQPEKSDPQQQKVEKVAAACRLLEQETPMTLEALARQAALSPFHFHRLF
KSVTGMTPKAWQQAWRARRLREALGQGAKITDAALSAGFPDNSSYYQQADAALGMTARQF
RRGGEDLDITWVCGDGPLGRCLVAESERGVCAVLPGESDALLCAELASLFPNARLHPGDE
AFRQRVAQVFSHLDDHRQPFSLPLDLRGTAFQLQVWQALRQIPAGETRSYRQVAQSIGRP
RAVRAVASACAANKLALVIPCHRVVREDGALSGYRWGTARKALLLAREASSEEK