Protein Info for BWI76_RS19805 in Klebsiella michiganensis M5al

Annotation: Bcr/CflA family multidrug efflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details transmembrane" amino acids 47 to 65 (19 residues), see Phobius details amino acids 77 to 98 (22 residues), see Phobius details amino acids 104 to 123 (20 residues), see Phobius details amino acids 135 to 160 (26 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 216 to 240 (25 residues), see Phobius details amino acids 252 to 272 (21 residues), see Phobius details amino acids 284 to 332 (49 residues), see Phobius details amino acids 344 to 364 (21 residues), see Phobius details amino acids 370 to 390 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 8 to 390 (383 residues), 502.9 bits, see alignment E=8.3e-155 PF07690: MFS_1" amino acids 16 to 359 (344 residues), 171.9 bits, see alignment E=1.9e-54 PF00083: Sugar_tr" amino acids 48 to 187 (140 residues), 44.7 bits, see alignment E=9.4e-16 TIGR00880: multidrug resistance protein" amino acids 52 to 188 (137 residues), 136.2 bits, see alignment E=8.3e-44

Best Hits

Swiss-Prot: 86% identical to BCR_ECOLI: Bicyclomycin resistance protein (bcr) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_24105)

MetaCyc: 86% identical to multidrug efflux pump Bcr (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-340; TRANS-RXN-44

Predicted SEED Role

"MFS family multidrug transport protein, bicyclomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6Z1 at UniProt or InterPro

Protein Sequence (398 amino acids)

>BWI76_RS19805 Bcr/CflA family multidrug efflux MFS transporter (Klebsiella michiganensis M5al)
MTAKQNSSLGIVFILGLLAMLMPLSIDMYLPALPVIAAQFNVPDGSAQMTLSTYILGFAL
GQLLYGPMADSLGRKPVILGGTLVFAAAAVACALSQTIDMLITMRFFHGLAAAAASVVIN
ALMRDIYPKEEFSRMMSFVMLVTTVAPLVAPMVGGAVLVWFSWHAIFWILALAALLASAM
IAFFISETLPPERRQKFHIRTTLGNFATLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
ININHVSPQHFGYYFALNVVFLFVMTMINSRFVRRVGALNMFRAGLWIQFAMAVWMVVCA
LFDVGFWALVFGVAAFIGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
LSMATFTTAWPMLISIAFCASSSILFYLYASRRRKAAR