Protein Info for BWI76_RS19755 in Klebsiella michiganensis M5al

Annotation: fructuronate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 PF01232: Mannitol_dh" amino acids 26 to 188 (163 residues), 163 bits, see alignment E=5.9e-52 PF08125: Mannitol_dh_C" amino acids 217 to 461 (245 residues), 329.1 bits, see alignment E=1.8e-102

Best Hits

Swiss-Prot: 79% identical to YEIQ_ECOLI: Uncharacterized oxidoreductase YeiQ (yeiQ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 86% identity to eae:EAE_24055)

MetaCyc: 58% identical to D-mannonate oxidoreductase (Escherichia coli K-12 substr. MG1655)
Fructuronate reductase. [EC: 1.1.1.57]

Predicted SEED Role

"D-mannonate oxidoreductase (EC 1.1.1.57)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.57)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.57

Use Curated BLAST to search for 1.1.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6X1 at UniProt or InterPro

Protein Sequence (488 amino acids)

>BWI76_RS19755 fructuronate reductase (Klebsiella michiganensis M5al)
MTTIATATLPEGVQLPQYDRSQLRSRIVHFGFGAFHRAHQALLTDRVLNAGGGDWGICEI
SLFSGDRLMSQLRQQDHLFTVLEKGAEGNQPIVVGAVHECLNAKLDSLAAIIEKFCEPQV
AIVSLTITEKGYCIDPASGLLDMTQPRIIHDLQNPTLPQSVPGILVEALSRRRQRELPPF
TVLSCDNIPNNGHVVKNAVLGMAEKRSPELAQWIAERVSFPGTMVDRIVPAATDESLAEI
SAALGVEDPCAISCEPFIQWVIEDNFVAGRPAWETAGVQMTDDVLPWEQMKLRMLNGSHS
FLAWLGYLAGHAHISDCMQDPIFRSAAYRLMLDEQAPTLSIQGVDLTAYADSLIERFSNP
ALKHRTWQIAMDGSQKLPQRMLDGIRVHLAQGSHWPLLALGVAGWMRYVSGIDDGGNAID
IRDPLADKIQKLVSASDEHQRVAALLTLEEIFGRDLPQDPQFVAYITAAWHQLAAFGARQ
AIAGTLNA