Protein Info for BWI76_RS19650 in Klebsiella michiganensis M5al

Annotation: DNA-binding transcriptional regulator GalS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF00356: LacI" amino acids 3 to 48 (46 residues), 72.6 bits, see alignment 3.5e-24 PF00532: Peripla_BP_1" amino acids 60 to 275 (216 residues), 78.2 bits, see alignment E=1.6e-25 PF13407: Peripla_BP_4" amino acids 62 to 278 (217 residues), 59.4 bits, see alignment E=8.7e-20 PF13377: Peripla_BP_3" amino acids 169 to 329 (161 residues), 113.3 bits, see alignment E=2.7e-36

Best Hits

Swiss-Prot: 86% identical to GALS_SALTY: HTH-type transcriptional regulator GalS (galS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_23945)

Predicted SEED Role

"Mgl repressor and galactose ultrainduction factor GalS, HTH-type transcriptional regulator" in subsystem Lactose and Galactose Uptake and Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6V3 at UniProt or InterPro

Protein Sequence (340 amino acids)

>BWI76_RS19650 DNA-binding transcriptional regulator GalS (Klebsiella michiganensis M5al)
MITIRDVARQAGVSVATVSRVLNNSALVSPDTREAVMKAVSLLGYRPNANAQALATQVSD
TIGVVVMDVSDAFFGALVKAVDTVAQQHHKYVLIGNSYHEAEKERNAIEVLIRQRCSALI
VHSKALSDAELGDFMEHMPGMVLINRIVPGYAHRCVGLDNVSGALMATRMLLNHGHQRIG
YLSSNHGIEDDDLRREGWFRALHEHGIVAPESWVGSGSPDMQGGEAAMVELLGRNLGLTA
VFAYNDSMAAGALTTLKDNGIAVPQHLSLIGFDDIPISRYTDPQLTTVRYPIMSMAKLAT
ELALQGAAGKLDREASHCFMPTLVRRHSVAQRQIVGPITN