Protein Info for BWI76_RS19425 in Klebsiella michiganensis M5al

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 TIGR00398: methionine--tRNA ligase" amino acids 8 to 536 (529 residues), 683.1 bits, see alignment E=2.9e-209 PF09334: tRNA-synt_1g" amino acids 8 to 397 (390 residues), 552.2 bits, see alignment E=1.8e-169 PF00133: tRNA-synt_1" amino acids 9 to 84 (76 residues), 44.6 bits, see alignment E=2.6e-15 PF19303: Anticodon_3" amino acids 415 to 541 (127 residues), 35.7 bits, see alignment E=2.4e-12 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 542 to 677 (136 residues), 154.1 bits, see alignment E=2e-49 PF01588: tRNA_bind" amino acids 581 to 674 (94 residues), 82.3 bits, see alignment E=6.7e-27

Best Hits

Swiss-Prot: 97% identical to SYM_KLEP7: Methionine--tRNA ligase (metG) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_23755)

MetaCyc: 94% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6G9 at UniProt or InterPro

Protein Sequence (677 amino acids)

>BWI76_RS19425 methionine--tRNA ligase (Klebsiella michiganensis M5al)
MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGHEVNFICADDAHGTPIM
LKAQQLGITPEQMIGEMSQEHQTDFAGFDISYDNYHSTHSDENRELSELIYTRLKENGFI
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIEPKSVVSG
ATPVMRESEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFG
FEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTTSFDEYWKKDSTAELYHFIGKDIVY
FHSLFWPAMLEGSSFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFDADSLRYYY
TAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGMLSAELADPQLYK
TFTDAATSIGEAWDSREFGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLQAICTM
GLNMFRVLMTWLKPVLPQLAARAEAFLNGELSWDAIQQPLLGHKVNAFKALYNRIEMKQV
DALVEASKEEVKALSAPVTGPLADDPIQETITFDDFAKVDMRIALIENAEFVDGSDKLLR
LTLDLGGEKRNVFSGIRSAYPDPQVLIGRLTVMVANLAPRKMRFGVSEGMVMAAGPGGKD
IFLLSPDSGAQPGQQVK