Protein Info for BWI76_RS19380 in Klebsiella michiganensis M5al

Annotation: hydroxyethylthiazole kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00694: hydroxyethylthiazole kinase
  • amino acids 17 to 252 (236 residues), 286.3 bits, E=1.1e-89
• PF02110: HK
  • amino acids 17 to 252 (236 residues), 315.2 bits, E=1.5e-98

Best Hits

Swiss-Prot: 84% identical to THIM_KLEP7: Hydroxyethylthiazole kinase (thiM) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 85% identity to eae:EAE_23680)

MetaCyc: 71% identical to hydroxyethylthiazole kinase (Escherichia coli K-12 substr. MG1655)
Hydroxyethylthiazole kinase. [EC: 2.7.1.50]; 2.7.1.50 [EC: 2.7.1.50]; Choline kinase. [EC: 2.7.1.50, 2.7.1.32]

Predicted SEED Role

"Hydroxyethylthiazole kinase (EC 2.7.1.50)" in subsystem Thiamin biosynthesis (EC 2.7.1.50)

MetaCyc Pathways

• thiamine diphosphate salvage II (5/5 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• thiamine diphosphate salvage IV (yeast) (5/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
• plasmalogen biosynthesis I (aerobic) (1/16 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.32 or 2.7.1.50

Sequence Analysis Tools

See A0A285B6H4 at UniProt or InterPro

Protein Sequence (257 amino acids)

>BWI76_RS19380 hydroxyethylthiazole kinase (Klebsiella michiganensis M5al)
MSDLLNPAHIAHLRHLFHRHSPLVHCMTNDVVQTFTANVLLAIGASPAMVIDPDEASQFA
AIADALLINVGTLTVERAAAMRAAAHSATQAGKPWALDPVAVGALTLRTEFCRELLALQP
AAVRGNASEIMALAGMSGGGRGVDTTDTAAAALPAAQALARQLNTVVAVTGEVDYITDGQ
RTRAVTGGDPLMTRVVGTGCALSAVVAAAAAMPGDRLENVAAACGLMKLAGGKAARQGGP
GSFTPAFLDALYAGGDL