Protein Info for BWI76_RS19155 in Klebsiella michiganensis M5al

Annotation: formate dehydrogenase-N subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1016 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 3 to 1015 (1013 residues), 1548.2 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 45 to 104 (60 residues), 57.9 bits, see alignment 1.2e-19 PF00384: Molybdopterin" amino acids 108 to 657 (550 residues), 106.3 bits, see alignment E=2.8e-34 PF01568: Molydop_binding" amino acids 904 to 1009 (106 residues), 48.5 bits, see alignment E=1.2e-16

Best Hits

Swiss-Prot: 70% identical to FDNG_ECOLI: Formate dehydrogenase, nitrate-inducible, major subunit (fdnG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 76% identity to dda:Dd703_1335)

MetaCyc: 69% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6A7 at UniProt or InterPro

Protein Sequence (1016 amino acids)

>BWI76_RS19155 formate dehydrogenase-N subunit alpha (Klebsiella michiganensis M5al)
MELSRRQFFRICAGGMAGTTVASLGFLPSFSAHAETRQYKLLKAKETRNNCTYCSVGCGI
LMYSFGDNAKNVRERIYHVEGDPDHPVSRGSLCPKGAGVLDYIHSDNRLRYPEYRAPGSD
KWTRMSWDEAIDRIARLMKDDRDANFIEKNALGVTVNRWTSTSMLCSSAASNETGILDGK
FTRALGMVSIDCQARLCHGPTVSALAPTFGRGAMTNNWVDIKNANVVLIMGGNAAEAHPV
GFKWVIEAKIKNNATVIVVDPRFNRSAAVADLYAPIRAGSDSAFLLGAIRYLIEHDRIQH
EYVRHYTNASMIVREDYDFDEGLFSGFDPQKRQYDKTSWNYELDANGLARRDDTWNHPRC
VWNLLKQHVERYTPELVNRLCGTSLHDFQRICELLASTSAANRTATILYALGWTHHTTGA
QTIRAAAMLQLLLGNIGMAGGGVNALRGHSNIQGYTDLGLLSTNLPGYMPLPSEKQVDYQ
SYISQITPAALGVNEVNYWQNTPKFFVSMMKSFWGDAATAENSWGYDWLPKWDRLYDVMT
QAELMAQGKINGYVVQGFNPLAAFPDKNKSARALAKLKYLVVIDPLVTESSNFWQNHGEM
NDVRPADIQTEVFRLPSSCFAEENGSIANSGRWLQWHWAAAEPPGEALHDGKILGRLFMR
LRDLYRQEGGANPAPVLNMSWDYHDPLDPQPEEVAREANGKALRDIVDEQGRVVVKKGQQ
LSSFAQLKDDGSTSSYCWVYCGCWTEQGNQMANRDNSDPYGLGCTPGWAWSWPANRRILY
NRASADPAGKPWDPQRSLLNWDGKRWTGMDVADYSQAAPNTNVGPFIMNPEGVARLFSLD
KLNDGPFPEHYEPVESPIGTNPLHPKVVSSPVARIYHDDLANMGKADEFPYVATTYSITE
LFRHWTKHARLNAIVQPEQFVEIGEALAKEKGIVAGDTVKVMSKRGFIKAKAVVTKRIQA
LTIDGRTVNTIGIPCHWGFEGATRKGFLANTLTPSVGDANSQTPEYKAFLVNVEKA