Protein Info for BWI76_RS19115 in Klebsiella michiganensis M5al

Annotation: outer membrane assembly protein AsmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF05170: AsmA" amino acids 2 to 190 (189 residues), 54.5 bits, see alignment E=9.5e-19 amino acids 225 to 508 (284 residues), 58.1 bits, see alignment E=7.9e-20 PF13502: AsmA_2" amino acids 356 to 539 (184 residues), 72.3 bits, see alignment E=4.6e-24

Best Hits

Swiss-Prot: 64% identical to ASMA_ECOLI: Protein AsmA (asmA) from Escherichia coli (strain K12)

KEGG orthology group: K07289, AsmA protein (inferred from 80% identity to kpe:KPK_1649)

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6L7 at UniProt or InterPro

Protein Sequence (615 amino acids)

>BWI76_RS19115 outer membrane assembly protein AsmA (Klebsiella michiganensis M5al)
MRRILTTLMILLAVVVAGLTALVLLVNPNDFRAYMVKEVADRSGYQLELNGPLRWHVWPQ
LSILSGRMTLTAPGAAEPVIRADNMRLDVGLLPLISHQLQVKQVMLKGAVIQLTPKTEAV
RSKGAPVVPHDNTLPQVPEDRGWSYDVQKLQIADSVLFFQHEDGEQVTVRDIRLQMEQDS
SHRATVDFSGRVNRDQRDLALSFTAQVQGGDYPHSLKADISQLNWQLRGAELPPEGISGQ
ASMQASWFEDAKKLSFDKLNLTANGSTVAGNGSVVLGQQPDWTLNLQAGTLNLDSLLSHP
SASDNNGVNQQGQSQARQLRPVIADNGIQQDYNSLRGFNARLSITAEQLQWRGMHFTQVK
SDISNQQGLLTIHQMQGNLDGGRLSLPGSLDARGETPRASFEPQLDNVEIGSILKAFNYS
INLTGKLSLTGEFSGGKIDADEFRRSWQGQAQIQMTDTRTEGLNFQQLVQQAVERSTNVQ
AQENYDNATRLDSVSTEFALDNGQVTLKGMQGQSELMALTGQGELNLLKEDCDMRFNVRV
LGGWKGESKLIDRLKQTAIPLRIYGNWQTLSYSLQVDQILRKQLQDEAKKRLSEWAERNK
NTQDGKDVKKLLDKL