Protein Info for BWI76_RS18935 in Klebsiella michiganensis M5al

Annotation: bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR01261: histidinol-phosphatase" amino acids 3 to 162 (160 residues), 257.1 bits, see alignment E=7e-81 TIGR01662: HAD hydrolase, family IIIA" amino acids 4 to 147 (144 residues), 89.3 bits, see alignment E=3.8e-29 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 5 to 144 (140 residues), 151 bits, see alignment E=3.4e-48 PF08645: PNK3P" amino acids 5 to 133 (129 residues), 25.4 bits, see alignment E=2.1e-09 PF13242: Hydrolase_like" amino acids 103 to 146 (44 residues), 25.3 bits, see alignment 2.3e-09 PF00475: IGPD" amino acids 196 to 337 (142 residues), 208.4 bits, see alignment E=9.9e-66

Best Hits

Swiss-Prot: 94% identical to HIS7_KLEP7: Histidine biosynthesis bifunctional protein HisB (hisB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 94% identity to kpe:KPK_1687)

MetaCyc: 88% identical to imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (Escherichia coli K-12 substr. MG1655)
Imidazoleglycerol-phosphate dehydratase. [EC: 4.2.1.19]; Histidinol-phosphatase. [EC: 4.2.1.19, 3.1.3.15]

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B683 at UniProt or InterPro

Protein Sequence (355 amino acids)

>BWI76_RS18935 bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase (Klebsiella michiganensis M5al)
MTQKFLFIDRDGTLISEPPVDFQVDRFDKLAFEPQVIPALLKLQQEGYKLVMITNQDGLG
TASLPQDEFDGPHNLMMQIFASQGVNFDEVLICPHLPADNCDCRKPKTRLVLPWLEQGVM
DTAHSYVIGDRATDIQLADNMGITGLRYDREQLDWPTICEQLTRRDRYAHVERITKETQV
DVKVWLDREGGSKISTGVGFFDHMLDQIATHGGFRLEVNVGGDLYIDDHHTVEDTGLALG
EALKLALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKADFTYQRVGDLSTEMVEH
FFRSLSYTMAVTLHLKTKGKNDHHRVESLFKAFGRTLRQAIRVQGDTLPSSKGVL