Protein Info for BWI76_RS18905 in Klebsiella michiganensis M5al

Annotation: putrescine/spermidine ABC transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 52 to 71 (20 residues), see Phobius details amino acids 93 to 116 (24 residues), see Phobius details amino acids 127 to 146 (20 residues), see Phobius details amino acids 157 to 179 (23 residues), see Phobius details amino acids 199 to 217 (19 residues), see Phobius details amino acids 237 to 259 (23 residues), see Phobius details amino acids 286 to 308 (23 residues), see Phobius details amino acids 336 to 355 (20 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details amino acids 395 to 413 (19 residues), see Phobius details amino acids 419 to 437 (19 residues), see Phobius details PF00324: AA_permease" amino acids 23 to 381 (359 residues), 122.1 bits, see alignment E=2.6e-39 PF13520: AA_permease_2" amino acids 46 to 411 (366 residues), 119 bits, see alignment E=2.6e-38

Best Hits

Swiss-Prot: 88% identical to PLAP_ECO57: Low-affinity putrescine importer PlaP (plaP) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 98% identity to esc:Entcl_1677)

MetaCyc: 88% identical to putrescine:H+ symporter PlaP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-69

Predicted SEED Role

"Putrescine importer"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6Q1 at UniProt or InterPro

Protein Sequence (452 amino acids)

>BWI76_RS18905 putrescine/spermidine ABC transporter (Klebsiella michiganensis M5al)
MSHNATPKTSRVELRKTLTLIPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVATAYAFALI
AILFTALSYGKLVRRFPSAGSAYTYAQKSISPAVGFMVGWSSLLDYLFMPMINILLAKIY
FEALVPSIPSWIFVVALVAFMTISNLRSIKTVANFNTLIVILQMGIVAVIVGLIIYGVMH
GEGAGTLTSTRPFWSEGAHVVPMITGATILCFSFLGFDGISSLSEETKDAERVIPKAIFL
TALIGGLIFIGASYFLQLYFPDISRFKDPDASQPEIMLYVAGKTFQWGVLIFSSVTVLAS
GMAAHAGVSRLMYVMGRDGVFPTRFFGYVHPKWRTPAWNVLLVGAIALLAIKFDLVTATA
LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFNYLILPVCGALTVGALWINLEESSM
VLGLIWGGIGLVYLACVTKSFRNPVPQYEDVA