Protein Info for BWI76_RS18895 in Klebsiella michiganensis M5al

Annotation: nitrogenase cofactor biosynthesis protein NifB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 23 to 446 (424 residues), 646.8 bits, see alignment E=7.9e-199 PF04055: Radical_SAM" amino acids 49 to 228 (180 residues), 71.7 bits, see alignment E=8.7e-24 PF02579: Nitro_FeMo-Co" amino acids 351 to 451 (101 residues), 64.6 bits, see alignment E=8.7e-22

Best Hits

Swiss-Prot: 100% identical to NIFB_KLEPN: FeMo cofactor biosynthesis protein NifB (nifB) from Klebsiella pneumoniae

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 86% identity to kpe:KPK_1697)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B660 at UniProt or InterPro

Protein Sequence (468 amino acids)

>BWI76_RS18895 nitrogenase cofactor biosynthesis protein NifB (Klebsiella michiganensis M5al)
MTSCSSFSGGKACRPADDSALTPLVADKAAAHPCYSRHGHHRFARMHLPVAPACNLQCNY
CNRKFDCSNESRPGVSSTLLTPEQAVVKVRQVAQAIPQLSVVGIAGPGDPLANIARTFRT
LELIREQLPDLKLCLSTNGLMLPDAVDRLLDVGVDHVTVTINTLDAEIAAQIYAWLWLDG
ERYSGREAGEILIARQLEGVRRLTAKGVLVKINSVLIPGINDSGMADVSRALRASGAFIH
NIMPLIARPEHGTVFGLNGQPEPDAETLAATRSRCGEVMPQMTHCHQCRADAIGMLGEDR
SQQFTQLPAPESLPAWLPILHQRAQLHASIATRGESEADDACLVAVASSRGDVIDCHFGH
ADRFYIYSLSAAGMVLVNERFTPKYCQGRDDCEPQDNAARFAAILELLADVKAVFCVRIG
HTPWQQLEQEGIEPCVDGAWRPVSEVLPAWWQQRRGSWPAALPHKGVA