Protein Info for BWI76_RS18890 in Klebsiella michiganensis M5al

Annotation: nif-specific transcriptional activator NifA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR01817: Nif-specific regulatory protein" amino acids 18 to 524 (507 residues), 755.3 bits, see alignment E=2e-231 PF13185: GAF_2" amino acids 37 to 176 (140 residues), 44.6 bits, see alignment E=5.9e-15 PF01590: GAF" amino acids 38 to 176 (139 residues), 46.5 bits, see alignment E=1.9e-15 PF00158: Sigma54_activat" amino acids 212 to 378 (167 residues), 243.2 bits, see alignment E=4.2e-76 PF14532: Sigma54_activ_2" amino acids 213 to 383 (171 residues), 68.8 bits, see alignment E=2.1e-22 PF00004: AAA" amino acids 236 to 364 (129 residues), 21.7 bits, see alignment E=8.2e-08 PF07728: AAA_5" amino acids 236 to 355 (120 residues), 30.7 bits, see alignment E=1e-10 PF02954: HTH_8" amino acids 481 to 515 (35 residues), 44 bits, see alignment 5.3e-15

Best Hits

Swiss-Prot: 100% identical to NIFA_KLEPN: Nif-specific regulatory protein (nifA) from Klebsiella pneumoniae

KEGG orthology group: K02584, Nif-specific regulatory protein (inferred from 92% identity to kpe:KPK_1698)

Predicted SEED Role

"Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6C3 at UniProt or InterPro

Protein Sequence (524 amino acids)

>BWI76_RS18890 nif-specific transcriptional activator NifA (Klebsiella michiganensis M5al)
MIHKSDSDTTVRRFDLSQQFTAMQRISVVLSRATEASKTLQEVLSVLHNDAFMQHGMICL
YDSQQEILSIEALQQTEDQTLPGSTQIRYRPGEGLVGTVLAQGQSLVLPRVADDQRFLDR
LSLYDYDLPFIAVPLMGPHSRPIGVLAAQPMARQEERLPACTRFLETVANLIAQTIRLMI
LPTSAAQAPQQSPRIERPRACTPSRGFGLENMVGKSPAMRQIMDIIRQVSRWDTTVLVRG
ESGTGKELIANAIHHNSPRAAAAFVKFNCAALPDNLLESELFGHEKGAFTGAVRQRKGRF
ELADGGTLFLDEIGESSASFQAKLLRILQEGEMERVGGDETLRVNVRIIAATNRHLEEEV
RLGHFREDLYYRLNVMPIALPPLRERQEDIAELAHFLVRKIAHSQGRTLRISDGAIRLLM
EYSWPGNVRELENCLERSAVLSESGLIDRDVILFNHRDNPPKALASSGPAEDGWLDNSLD
ERQRLIAALEKAGWVQAKAARLLGMTPRQVAYRIQIMDITMPRL