Protein Info for BWI76_RS18875 in Klebsiella michiganensis M5al

Annotation: nitrogen fixation protein NifM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 TIGR02933: nitrogen fixation protein NifM" amino acids 6 to 258 (253 residues), 405.1 bits, see alignment E=8e-126 PF13145: Rotamase_2" amino acids 107 to 233 (127 residues), 41.7 bits, see alignment E=2.9e-14 PF13616: Rotamase_3" amino acids 124 to 217 (94 residues), 40.4 bits, see alignment E=5.9e-14 PF00639: Rotamase" amino acids 131 to 219 (89 residues), 64.5 bits, see alignment E=2.2e-21

Best Hits

Swiss-Prot: 100% identical to NIFM_KLEPN: Putative peptidyl-prolyl cis-trans isomerase NifM (nifM) from Klebsiella pneumoniae

KEGG orthology group: K03769, peptidyl-prolyl cis-trans isomerase C [EC: 5.2.1.8] (inferred from 75% identity to kva:Kvar_1590)

Predicted SEED Role

"Protein nifM"

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B673 at UniProt or InterPro

Protein Sequence (266 amino acids)

>BWI76_RS18875 nitrogen fixation protein NifM (Klebsiella michiganensis M5al)
MNPWQRFARQRLARSRWNRDPAALDPADTPAFEQAWQRQCHMEQTIVARVPEGDIPAALL
ENIAASLAIWLDEGDFAPPERAAIVRHHARLELAFADIARQAPQPDLSTVQAWYLRHQTQ
FMRPEQRLTRHLLLTVDNDREAVHQRILGLYRQINASRDAFAPLAQRHSHCPSALEEGRL
GWISRGLLYPQLETALFSLAENALSLPIASELGWHLLWCEAIRPAAPMEPQQALESARDY
LWQQSQQRHQRQWLEQMISRQPGLCG