Protein Info for BWI76_RS18810 in Klebsiella michiganensis M5al

Annotation: nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 TIGR01287: nitrogenase iron protein" amino acids 4 to 278 (275 residues), 483.4 bits, see alignment E=9.2e-150 PF02374: ArsA_ATPase" amino acids 4 to 46 (43 residues), 26.3 bits, see alignment 1.3e-09 PF00142: Fer4_NifH" amino acids 4 to 275 (272 residues), 433.2 bits, see alignment E=1.3e-133 PF01656: CbiA" amino acids 8 to 228 (221 residues), 49.7 bits, see alignment E=1.1e-16 PF10609: ParA" amino acids 9 to 59 (51 residues), 30 bits, see alignment 1.1e-10 PF13614: AAA_31" amino acids 10 to 74 (65 residues), 43.1 bits, see alignment E=1.4e-14

Best Hits

Swiss-Prot: 100% identical to NIFH_KLEPN: Nitrogenase iron protein (nifH) from Klebsiella pneumoniae

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 98% identity to kva:Kvar_1603)

MetaCyc: 100% identical to [FeMo]-nitrogenase complex nitrogenase reductase component monomer (Klebsiella pneumoniae)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) reductase and maturation protein NifH" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B637 at UniProt or InterPro

Protein Sequence (293 amino acids)

>BWI76_RS18810 nitrogenase iron protein (Klebsiella michiganensis M5al)
MTMRQCAIYGKGGIGKSTTTQNLVAALAEMGKKVMIVGCDPKADSTRLILHAKAQNTIME
MAAEVGSVEDLELEDVLQIGYGDVRCAESGGPEPGVGCAGRGVITAINFLEEEGAYEDDL
DFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYAKSGKVRL
GGLICNSRQTDREDELIIALAEKLGTQMIHFVPRDNIVQRAEIRRMTVIEYDPACKQANE
YRTLAQKIVNNTMKVVPTPCTMDELESLLMEFGIMEEEDTSIIGKTAAEENAA