Protein Info for BWI76_RS18780 in Klebsiella michiganensis M5al

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00768: Peptidase_S11" amino acids 28 to 262 (235 residues), 338.4 bits, see alignment E=3.5e-105 PF13354: Beta-lactamase2" amino acids 39 to 239 (201 residues), 35.1 bits, see alignment E=1.4e-12 PF07943: PBP5_C" amino acids 282 to 372 (91 residues), 81 bits, see alignment E=8.5e-27

Best Hits

Swiss-Prot: 82% identical to DACD_ECOLI: D-alanyl-D-alanine carboxypeptidase DacD (dacD) from Escherichia coli (strain K12)

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 92% identity to kpn:KPN_02468)

MetaCyc: 82% identical to D-alanyl-D-alanine carboxypeptidase DacD (Escherichia coli K-12 substr. MG1655)
Beta-lactamase. [EC: 3.5.2.6]; Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.5.2.6, 3.4.16.4]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.5.2.6

Use Curated BLAST to search for 3.4.16.4 or 3.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6E2 at UniProt or InterPro

Protein Sequence (388 amino acids)

>BWI76_RS18780 D-alanyl-D-alanine carboxypeptidase (Klebsiella michiganensis M5al)
MKGRLFIAVSLLAASVSCAFAANDFPVNVTPPSIQAGSWVLMDYTTGQILTAGNEHQQRN
PASLTKLMTGYVVDRAIDSHRITFDDIVTVGKDAWAQGNPVFNGSSLMFLKPGDRVSVRD
LSRGLIVDSGNDACVALADYVAGGQPQFVALMNSYVEKLHLQDTHFETVHGLDAPGQHSS
AYDLAVLSRAIIHGEPDVYHMYSQKSLTWNGITQQNRNGLLWDKTMNVDGLKTGHTSGAG
FNLIASAVDGQRRLIAVVMGADSPKGREQQAAKLLHWGQQNFDTVQVLQDGKQVGVERIW
YGDKEQIKLGTDQDFWLALPKSEVARIKAKYVLDNKVLEAPIAAHQRVGEISLYDGDKVV
AHWPLVTLESVGKGGMLSRFSDYLHQKL