Protein Info for BWI76_RS18720 in Klebsiella michiganensis M5al

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 transmembrane" amino acids 25 to 49 (25 residues), see Phobius details amino acids 60 to 84 (25 residues), see Phobius details amino acids 96 to 116 (21 residues), see Phobius details amino acids 122 to 149 (28 residues), see Phobius details amino acids 161 to 184 (24 residues), see Phobius details amino acids 196 to 215 (20 residues), see Phobius details amino acids 253 to 277 (25 residues), see Phobius details amino acids 290 to 312 (23 residues), see Phobius details amino acids 320 to 338 (19 residues), see Phobius details amino acids 344 to 365 (22 residues), see Phobius details amino acids 385 to 406 (22 residues), see Phobius details amino acids 412 to 434 (23 residues), see Phobius details PF00083: Sugar_tr" amino acids 28 to 414 (387 residues), 84 bits, see alignment E=1.1e-27 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 30 to 427 (398 residues), 496.3 bits, see alignment E=3.6e-153 PF07690: MFS_1" amino acids 57 to 302 (246 residues), 70.2 bits, see alignment E=1.6e-23 amino acids 268 to 440 (173 residues), 55.2 bits, see alignment E=5.6e-19

Best Hits

Swiss-Prot: 86% identical to SHIA_ECOLI: Shikimate transporter (shiA) from Escherichia coli (strain K12)

KEGG orthology group: K08172, MFS transporter, MHS family, shikimate and dehydroshikimate transport protein (inferred from 90% identity to kpe:KPK_1730)

MetaCyc: 86% identical to shikimate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-27

Predicted SEED Role

"Shikimate transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6A8 at UniProt or InterPro

Protein Sequence (444 amino acids)

>BWI76_RS18720 MFS transporter (Klebsiella michiganensis M5al)
MDSTLISQRPGKEACSLNRARRAAWGSFAGAVVDWYDFLLYGITAALVFNREFFPQISPA
MGTLAAFATFGVGFLFRPLGGIIFGHFGDRLGRKRMLMLTVWMMGIATACIGLLPSFSQI
GWWAPVLLVLLRAVQGFAVGGEWGGAALLAVESAPKNKKAFYSSGVQVGYGVGLLLSTGL
VSLISASTTDEQFLSWGWRLPFLFSVVLVLAALWIRNGMAESAEFEQQQREQQGQPKKKR
LPVVEALFRHPGAFLMIIGLRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGAI
SCLTIPCFAWLADRFGRRRVYITGALIGTLSGYPFFMALESQSYFWILFFALMLANIAHD
MVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALVTLSGGAWHSVAIYL
TAGCLLSALTAMLMKPSRHDEEPQ