Protein Info for BWI76_RS18685 in Klebsiella michiganensis M5al

Annotation: 6-phospho-alpha-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 PF02056: Glyco_hydro_4" amino acids 7 to 185 (179 residues), 201.2 bits, see alignment E=1.1e-63 PF11975: Glyco_hydro_4C" amino acids 196 to 417 (222 residues), 194.3 bits, see alignment E=2.6e-61

Best Hits

Swiss-Prot: 68% identical to MALH_FUSMR: Maltose-6'-phosphate glucosidase (malH) from Fusobacterium mortiferum

KEGG orthology group: None (inferred from 89% identity to kpn:KPN_01682)

MetaCyc: 65% identical to maltose-6'-phosphate glucosidase (Bacillus subtilis subtilis 168)
Maltose-6'-phosphate glucosidase. [EC: 3.2.1.122]

Predicted SEED Role

"Maltose-6'-phosphate glucosidase (EC 3.2.1.122)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Uptake and Utilization (EC 3.2.1.122)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.122

Use Curated BLAST to search for 3.2.1.122

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B635 at UniProt or InterPro

Protein Sequence (442 amino acids)

>BWI76_RS18685 6-phospho-alpha-glucosidase (Klebsiella michiganensis M5al)
MKNKFVVTIAGGGSTYTPGIVMMLLENMDRFPLSEIRLYDNHRDRQSVIGEACAILVAER
YPQVAFSFTTDPQEAFTGVDFVMAHIRVGLYEMREKDEKIPLKHGVIGQETCGPGGIAYG
MRSIAGVLELVDYMETYSPGAWMLNYSNPAAIVAEATRRLRPTAKIINICDMPVAIEGLF
ADILGLKSRKEMNVRYFGLNHFGWWTSITDKAGNDLMPALKNHVAELGYCSPKEDFQHKA
PSWIETFKKVKDVFALDTDTLPNTYLKYYFYADYEVAHSDPDFTRANEVIAGREKEVFDM
AREIVARQSGKAAHFHAGAHATFIVDLACAIAFNTQERMLLIVENNGAIANFDDTAMVEV
PCLVGVNGPEPLAMGKIPLFQKGMMEQQVAVEKLVVDAWVEGSYQKLWQAMTLSKTVPSA
SVAKAILDELIVANRGYWPELR