Protein Info for BWI76_RS18370 in Klebsiella michiganensis M5al

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF00128: Alpha-amylase" amino acids 31 to 367 (337 residues), 74 bits, see alignment E=1.7e-24

Best Hits

Swiss-Prot: 84% identical to AMY2_ECOLI: Cytoplasmic alpha-amylase (amyA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 91% identity to eae:EAE_23060)

MetaCyc: 84% identical to alpha-amylase (Escherichia coli K-12 substr. MG1655)
Alpha-amylase. [EC: 3.2.1.1]

Predicted SEED Role

"Cytoplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5Y5 at UniProt or InterPro

Protein Sequence (495 amino acids)

>BWI76_RS18370 alpha-amylase (Klebsiella michiganensis M5al)
MKNPTLLQCFHWYYPTGGELWREVEALAPNLNEIGINMIWLPPAYKGASGGYSVGYDCYD
LFDLGEFDQKGSVATKYGDKTQLLAAIAALRQHDIAVLLDVVVNHKMGADEKEPVRIQRV
DQNDRTQIADEIVECEAWTRYTFPVRAGQYSEFIWDYKCFSGIDHIENPDEDGVFKIVND
YTGEGWNDQVDDEMGNFDYLMGENIDFRNRAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
PAWFYKEWIDHVQEVATKPLFIVAEYWSHEVDKLQQYIAMVDGKTMLFDAPLQMKFHEAS
RQGRDYDMSQIFSGTLVEADPFHTVTLVTNHDTQPLQALEAPVEAWFKPLAYALILLREN
GVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELIDARQRFAHGVQTLWFDHPNCI
AFSRSGTEDDPGCVVVLSNGDDGEKTLPLGENYRNKRWRDFLGNREEVIETDDEGNGIFT
CNGGSVSVWVIEEVL