Protein Info for BWI76_RS18250 in Klebsiella michiganensis M5al

Annotation: arabinose ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00532: Peripla_BP_1" amino acids 25 to 319 (295 residues), 294.7 bits, see alignment E=8e-92 PF13407: Peripla_BP_4" amino acids 29 to 292 (264 residues), 70.7 bits, see alignment E=1.6e-23

Best Hits

Swiss-Prot: 92% identical to ARAF_ECOLI: L-arabinose-binding periplasmic protein (araF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_22960)

MetaCyc: 92% identical to arabinose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose-binding periplasmic protein precursor AraF (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5N7 at UniProt or InterPro

Protein Sequence (326 amino acids)

>BWI76_RS18250 arabinose ABC transporter substrate-binding protein (Klebsiella michiganensis M5al)
MHKFTKALAAIGLAAVMSQSAMAETLKLGFLVKQPEEPWFQTEWKFADKAGKDLGFDVIK
IAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGAAIVAKARGYDMKVITVDDQFVNAKG
KPMDSVPLVMMAASEIGARQGQELYKEMQKRGWDVKDTAVMAITADELDTARRRTTGSID
ALKAAGFPEQQIYRVPTKSNDIPGAFDAGNSMLVQHPNVKHWLIVGMNDNTVLGGVRATE
GQGFKAPDVIGIGINGVDAVNELSKAQATGFYGSLLPSPDIHGYKTSEMLYNWVTKDVEP
PKFTAVTDVVLITRDNFKEELAKKGL