Protein Info for BWI76_RS18235 in Klebsiella michiganensis M5al

Annotation: trehalose-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 TIGR00685: trehalose-phosphatase" amino acids 16 to 243 (228 residues), 225 bits, see alignment E=1.4e-70 TIGR01484: HAD hydrolase, family IIB" amino acids 17 to 210 (194 residues), 72.5 bits, see alignment E=5.1e-24 PF02358: Trehalose_PPase" amino acids 18 to 231 (214 residues), 171.4 bits, see alignment E=2.6e-54

Best Hits

Swiss-Prot: 68% identical to OTSB_SALTY: Trehalose-phosphate phosphatase (otsB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 73% identity to eae:EAE_22945)

MetaCyc: 63% identical to trehalose-6-phosphate phosphatase (Escherichia coli K-12 substr. MG1655)
Trehalose-phosphatase. [EC: 3.1.3.12]

Predicted SEED Role

"Trehalose-6-phosphate phosphatase (EC 3.1.3.12)" in subsystem Trehalose Biosynthesis (EC 3.1.3.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5Z0 at UniProt or InterPro

Protein Sequence (267 amino acids)

>BWI76_RS18235 trehalose-phosphatase (Klebsiella michiganensis M5al)
MADQIYVPPALTGNCAFFFDLDGTLAGIKPHPDQVVIPADVLQTLRQLVQQHNGAVALIS
GRSMVELDELTRPYRLPLAGVHGAERRDINGKTHIVSLPDSLLKALSAELTAALDALPGC
ELESKGMAFALHYRQAPEHQHAVLALAQAVVQRHPILALQPGKCVVEIKPRGVNKGEAIA
AFMQEAPFRGRKPVFVGDDLTDEAGFSVVNQLSGVSVKVGGGETQARWRLPDVAAVHLWI
SNIANHGQQPDALTDRRDGYESLSRSI