Protein Info for BWI76_RS18230 in Klebsiella michiganensis M5al

Annotation: trehalose-6-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details TIGR02400: alpha,alpha-trehalose-phosphate synthase (UDP-forming)" amino acids 3 to 452 (450 residues), 604.1 bits, see alignment E=7.8e-186 PF00982: Glyco_transf_20" amino acids 17 to 452 (436 residues), 493.1 bits, see alignment E=4.4e-152

Best Hits

Swiss-Prot: 86% identical to OTSA_KLEP7: Trehalose-6-phosphate synthase (otsA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00697, alpha,alpha-trehalose-phosphate synthase (UDP-forming) [EC: 2.4.1.15] (inferred from 86% identity to kpn:KPN_02392)

MetaCyc: 84% identical to trehalose-6-phosphate synthase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalose-phosphate synthase (UDP-forming). [EC: 2.4.1.15]

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5S3 at UniProt or InterPro

Protein Sequence (474 amino acids)

>BWI76_RS18230 trehalose-6-phosphate synthase (Klebsiella michiganensis M5al)
MSRLVVVSNRIAPPDDKKASAGGLAVGIMGALKAAGGLWFGWSGEIGDDQQPLKKVTQGN
ITWASFNLSEQDHDEYYNRFSNAVLWPAFHYRLDLVDFQREAWEGYQRVNAKLADKLLPL
IEADDIVWIHDYHLLPLASELRQRGVNNRIGFFLHIPFPTPEVFNALPPHAELLEQLCDY
DLLGFQTESDRLAFLDCVSMQTRLTTRGGKKHVAWGKPFQTEVYPIGIDPEEIARSAKGP
LPPKLAQLKSELKSVQNIFSVERLDYSKGLPERFLAYEALLENYPHHHGKIRYTQIAPTS
RGDVQAYQDIRHQLETAAGRINGRYGQLGWTPLYYLNQHFERKLLMKIFRYSDVGLVTPL
RDGMNLVAKEYVAAQDPQNPGVLVLSQFAGAANELPTALIVNPYDRDEVAAALDQALTMP
LAERIARHSAMLAVIKDNDIHRWQERFIADLKEIEPGSEESHLARKVATFPKLA