Protein Info for BWI76_RS18195 in Klebsiella michiganensis M5al

Annotation: tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00452: tRNA (mo5U34)-methyltransferase
  • amino acids 7 to 320 (314 residues), 492.6 bits, E=1.8e-152
• PF08003: Methyltransf_9
  • amino acids 7 to 322 (316 residues), 562.8 bits, E=5.2e-173
• PF13489: Methyltransf_23
  • amino acids 113 to 270 (158 residues), 46.4 bits, E=1.1e-15
• PF13847: Methyltransf_31
  • amino acids 123 to 228 (106 residues), 34.7 bits, E=4.6e-12
• PF13649: Methyltransf_25
  • amino acids 126 to 220 (95 residues), 32 bits, E=5e-11
• PF08241: Methyltransf_11
  • amino acids 127 to 224 (98 residues), 30.2 bits, E=1.8e-10

Best Hits

Swiss-Prot: 94% identical to CMOB_KLEP3: tRNA U34 carboxymethyltransferase (cmoB) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K15257, tRNA (mo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 94% identity to kpu:KP1_3516)

MetaCyc: 85% identical to tRNA U34 carboxymethyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-7067

Predicted SEED Role

"tRNA (5-methoxyuridine) 34 synthase"

MetaCyc Pathways

• 5-(methoxycarbonylmethoxy)uridine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See A0A285B5U1 at UniProt or InterPro

Protein Sequence (338 amino acids)

>BWI76_RS18195 tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB (Klebsiella michiganensis M5al)
MIDFSNFYQLIAKSPLSHWLETLPAQVAAWQRDALHGKYREWERAVEFLPEFAPYRLDLL
HSVTAESETPLGEGQRLRIENLLKNLMPWRKGPFSLYGVNIDTEWRSDWKWERVLPHLSD
LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG
IEQLPALNAFDTVFSMGVLYHRRSPLDHLWQLKDQLVPGGELVLETLVVEGDENTVLVPG
DRYAQMRNVYFIPSAAALKQWLEKCGFVDVRIVDACVTSTDEQRRTEWMTTESLADFLDP
RDSSKTVEGYPAPLRAVIIASKPETQQSLAAKKNRSQG