Protein Info for BWI76_RS18190 in Klebsiella michiganensis M5al

Annotation: carboxy-S-adenosyl-L-methionine synthase CmoA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 TIGR00740: tRNA (cmo5U34)-methyltransferase" amino acids 4 to 242 (239 residues), 432.6 bits, see alignment E=2.2e-134 PF00891: Methyltransf_2" amino acids 53 to 166 (114 residues), 33.2 bits, see alignment E=1e-11 PF01209: Ubie_methyltran" amino acids 55 to 166 (112 residues), 22.3 bits, see alignment E=2.3e-08 PF13847: Methyltransf_31" amino acids 57 to 164 (108 residues), 35.2 bits, see alignment E=2.8e-12 PF13649: Methyltransf_25" amino acids 60 to 158 (99 residues), 52.1 bits, see alignment E=2.6e-17 PF08241: Methyltransf_11" amino acids 62 to 162 (101 residues), 40.7 bits, see alignment E=9.2e-14 PF08242: Methyltransf_12" amino acids 62 to 160 (99 residues), 39.8 bits, see alignment E=1.9e-13

Best Hits

Swiss-Prot: 93% identical to CMOA_KLEP3: Carboxy-S-adenosyl-L-methionine synthase (cmoA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_22895)

MetaCyc: 90% identical to carboxy-S-adenosyl-L-methionine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-7066

Predicted SEED Role

"tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5N4 at UniProt or InterPro

Protein Sequence (247 amino acids)

>BWI76_RS18190 carboxy-S-adenosyl-L-methionine synthase CmoA (Klebsiella michiganensis M5al)
MSHRDTLFSAPIASLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPDTQV
YDLGCSLGAATLSVRRNIAHSGCKIIAIDNSPAMVERCRRHIDAYKAPTPVEVIEGDIRH
VKIENASMVVLNFTIQFLEPGERQAILDKVYQGLNPGGALVLSEKFSFEDDNVGELLFNM
HHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLRKAGFVHAELWFQCFNFGSLIA
VKSGDPA