Protein Info for BWI76_RS18150 in Klebsiella michiganensis M5al

Annotation: Holliday junction branch migration protein RuvA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 203 PF01330: RuvA_N" amino acids 1 to 62 (62 residues), 78 bits, see alignment E=1.1e-25 TIGR00084: Holliday junction DNA helicase RuvA" amino acids 1 to 203 (203 residues), 237.1 bits, see alignment E=5.7e-75 PF14520: HHH_5" amino acids 72 to 130 (59 residues), 55.3 bits, see alignment E=1.8e-18 PF07499: RuvA_C" amino acids 158 to 201 (44 residues), 57.4 bits, see alignment 3.9e-19

Best Hits

Swiss-Prot: 94% identical to RUVA_KLEP3: Holliday junction ATP-dependent DNA helicase RuvA (ruvA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K03550, holliday junction DNA helicase RuvA (inferred from 94% identity to kpn:KPN_02376)

MetaCyc: 92% identical to Holliday junction branch migration complex subunit RuvA (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvA" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5L4 at UniProt or InterPro

Protein Sequence (203 amino acids)

>BWI76_RS18150 Holliday junction branch migration protein RuvA (Klebsiella michiganensis M5al)
MIGRLRGIILEKQPPLVLLETGGVGYEVHLPMTCFYELPDVGKEAIIFTHFIVREDAQLL
YGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREELAALVKLPGIGKKT
AERLIVEMKDRFKGLHGDLFTPAADLVLTSPAGPSDDDAEQEAVAALVALGYKPQEASRM
VSKIVRPDANSETLIREALRAAL