Protein Info for BWI76_RS18115 in Klebsiella michiganensis M5al
Annotation: pyruvate kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to KPYK2_SALTY: Pyruvate kinase II (pykA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K00873, pyruvate kinase [EC: 2.7.1.40] (inferred from 98% identity to kpe:KPK_1919)MetaCyc: 96% identical to pyruvate kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyruvate kinase. [EC: 2.7.1.40]
Predicted SEED Role
"Pyruvate kinase (EC 2.7.1.40)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.1.40)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
- superpathway of N-acetylneuraminate degradation (22/22 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
- superpathway of hexitol degradation (bacteria) (18/18 steps found)
- superpathway of glucose and xylose degradation (17/17 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
- mixed acid fermentation (16/16 steps found)
- superpathway of anaerobic sucrose degradation (18/19 steps found)
- glycolysis I (from glucose 6-phosphate) (13/13 steps found)
- homolactic fermentation (12/12 steps found)
- glycolysis II (from fructose 6-phosphate) (11/11 steps found)
- glycolysis III (from glucose) (11/11 steps found)
- heterolactic fermentation (16/18 steps found)
- Entner-Doudoroff pathway I (9/9 steps found)
- Bifidobacterium shunt (13/15 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- Rubisco shunt (9/10 steps found)
- glycolysis IV (8/10 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (7/9 steps found)
- glycerol degradation to butanol (12/16 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
- glycolysis VI (from fructose) (8/11 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- glycolysis V (Pyrococcus) (7/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
- Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
- Purine metabolism
- Pyruvate metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.40
Use Curated BLAST to search for 2.7.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B5K9 at UniProt or InterPro
Protein Sequence (480 amino acids)
>BWI76_RS18115 pyruvate kinase (Klebsiella michiganensis M5al) MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGTAEDHQIRADKVREIAAK LGRHVAILGDLQGPKIRVSTFREGKIFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD VVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA DIVTAAKIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCDQDAMDDVI LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRSVITATQMMESMITNPMPTRAE VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSLNVSKHRLDIQFDN VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFALSRHERTLNLTALY RGVTPVHFDSVNDGVAAAHDAVTLLRDKGYLVTGDLVIVTQGDVMSTIGSTNTTRILTVE