Protein Info for BWI76_RS17755 in Klebsiella michiganensis M5al

Annotation: disulfide bond formation protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 176 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 45 to 64 (20 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 145 to 163 (19 residues), see Phobius details PF02600: DsbB" amino acids 12 to 159 (148 residues), 153.5 bits, see alignment E=2.8e-49

Best Hits

Swiss-Prot: 85% identical to DSBB_SALPA: Disulfide bond formation protein B (dsbB) from Salmonella paratyphi A (strain ATCC 9150 / SARB42)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_22120)

MetaCyc: 80% identical to protein thiol:quinone oxidoreductase DsbB (Escherichia coli K-12 substr. MG1655)
RXN-19950 [EC: 1.8.5.9]

Predicted SEED Role

"Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.5.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5E5 at UniProt or InterPro

Protein Sequence (176 amino acids)

>BWI76_RS17755 disulfide bond formation protein B (Klebsiella michiganensis M5al)
MLRYLNQCSRGRGAWLLMALTAFILELVALWFQHVMLLQPCVLCIYERCALFGVMGAGIV
GAIAPKTPLRYVAIVIWLYSALRGLQLSWEHTMIQLHPSPFQTCDFAARFPTWLPLDKWL
PQVFVATGDCSVRQWEFLTLEMPQWLVGIFAAYLVVGVLVLIAQPFKPKKRDLFGR