Protein Info for BWI76_RS17605 in Klebsiella michiganensis M5al

Annotation: phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to PCKA_SHIDS: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Shigella dysenteriae serotype 1 (strain Sd197)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 92% identity to kpn:KPN_02285)

MetaCyc: 73% identical to phosphoenolpyruvate carboxykinase (ATP) (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate carboxykinase (ATP). [EC: 4.1.1.49]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

gluconeogenesis I (13/13 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.49

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5B6 at UniProt or InterPro

Protein Sequence (546 amino acids)

>BWI76_RS17605 phosphoenolpyruvate carboxykinase (ATP) (Klebsiella michiganensis M5al)
MSRIESVKDALLSLGIQESSELFYNPDYDLLIAHETSPELTGAARGVMTESGAVAVDTGI
FTGRSPRDKYIVRDELTRDTVWWADSGLGRNDNKPLSSEIWADLKALVAGQLSGKKLYVV
DAWCGASPATRLGVRFVTEVAWQAHFVKNMFIAPSAEELAEFTPDFVVLNGAGCTNDKWQ
EQRLNSENFVAFNLSEKIQLIGGTWYGGEMKKGLFSVMNYLLPQQGIASMHCSANRGEAG
DVALFFGLSGTGKTTLSTDPLRQLIGDDEHGWDDDGVFNFEGGCYAKTINLDPQAEPEIY
RAIRRNALLENVVVRADGSVDYADGSKTENTRVSYPLSHIENIVKPVSRAGHPSKVIFLA
ADAFGVLPPVSRLTTEQMQYHFLSGFTSKLAGTERGITQPTPTFSACYGAAFLLLHPTQY
ASVLAAKMAEAGAEAWLVNTGWNGEGKRLSLRDTRNIISAILNGTTGELREETVPVFGLA
IPQSVPGVDSALLDPRSGWPSADKWQEKAESLAQLFMDNFKQYSDTDAGARLALAGPTLA
KSAVTA