Protein Info for BWI76_RS17570 in Klebsiella michiganensis M5al

Annotation: iron-sulfur-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 7 to 57 (51 residues), 25.6 bits, E=9.6e-10
• PF01799: Fer2_2
  • amino acids 74 to 147 (74 residues), 103.9 bits, E=4.1e-34

Best Hits

Swiss-Prot: 48% identical to CUTC_SULAC: Glyceraldehyde dehydrogenase small chain (cutC) from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

KEGG orthology group: K03518, carbon-monoxide dehydrogenase small subunit [EC: 1.2.99.2] (inferred from 91% identity to cro:ROD_33891)

MetaCyc: 54% identical to glycolaldehyde oxidoreductase small subunit (Saccharolobus solfataricus P2)
1.2.99.-

Predicted SEED Role

"Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purines degradation in plants (15/18 steps found)
• ureide biosynthesis (7/7 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• L-arabinose degradation IV (4/8 steps found)
• D-xylose degradation IV (3/7 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)
• superpathway of pentose and pentitol degradation (21/42 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Purine metabolism
• Trinitrotoluene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4 or 1.2.99.2

Sequence Analysis Tools

See A0A285B5M9 at UniProt or InterPro

Protein Sequence (152 amino acids)

>BWI76_RS17570 iron-sulfur-binding protein (Klebsiella michiganensis M5al)
MNHIELKVNGIRIARDVKDNVRLIDFLREELKLTGTKEGCSVGECGACTVIMDGKSVCSC
LLLAAQCNGVEVTTVEALHHDPVGKKLQNAFIRHGGVQCGFCTPGVLMSAKALLDTNPNP
SDDELKEALEGNICRCTGYQPIITSIKAVLKS